Study on detection of allergenic substances (egg and milk) in processed meat products and frozen foods

• Published in: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) Vol. 46; no. 4; pp. 139 - 147
• Main Authors: Watanabe, H.(Kanagawa-ken. Inst. of Public Health, Chigasaki (Japan)), Kai, S, Mitani, T, Yokoyama, H, Kishi, M
• Format: Journal Article
• Language: Japanese; English
• Published: Japan Japanese Society for Food Hygiene and Safety 01.08.2005
• Subjects: ALERGENOS; ALIMENT SURGELE POUR HOMME; ALIMENTOS; ALIMENTOS CONGELADOS; ALLERGENE; allergenic substances; ALLERGENS; Allergens - analysis; ANALYTICAL METHODS; Animals; Blotting, Western - methods; egg; EGGS; Eggs - analysis; ELISA; ELISA method; Enzyme-Linked Immunosorbent Assay - methods; Food Analysis - methods; Food Contamination; Food Handling; FOODS; FROZEN FOODS; Frozen Foods - analysis; HUEVOS; IMMUNOBLOTTING; LAIT; LECHE; MEAT PRODUCTS; Meat Products - analysis; MILK; OEUF; PRODUCTOS DE LA CARNE; PRODUIT ALIMENTAIRE; PRODUIT CARNE; TECHNIQUE ANALYTIQUE; TECNICAS ANALITICAS; TEST ELISA; Western blot analysis
• ISSN: 0015-6426; 1882-1006
• DOI: 10.3358/shokueishi.46.139

Allergenic substances (egg and miik) were measured in processed meat products and frozen foods of which the milk and egg ingredient ratios and manufacturing processes were clearly identified. An ovomucoid ELISA kit gave good results in the detection of egg ingredients. With an ovalbumin ELISA kit and egg ELISA kit, results for 6 of 16 foods were negative, but better results were obtained when a protein denaturant was added to the extraction buffer. Occurrence of contamination in the egg ingredient manufacturing line was confirmed in frozen foods. For milk ingredients, good results were obtained by ELISA using two kinds of kits (a casein ELISA kit and milk ELISA kit) described as being suitable for detection of allergenic substances. Allergenic substances were identified by Western blot analysis in all of the foods containing egg and milk ingredients.