Control of ribosomal protein synthesis by the Microprocessor complex

Ribosome biogenesis in eukaryotes requires the coordinated production and assembly of 80 ribosomal proteins and four ribosomal RNAs (rRNAs), and its rate must be synchronized with cellular growth. Here, we showed that the Microprocessor complex, which mediates the first step of microRNA processing,...

Full description

Saved in:
Bibliographic Details
Published inScience signaling Vol. 14; no. 671
Main Authors Jiang, Xuan, Prabhakar, Amit, Van der Voorn, Stephanie M, Ghatpande, Prajakta, Celona, Barbara, Venkataramanan, Srivats, Calviello, Lorenzo, Lin, Chuwen, Wang, Wanpeng, Black, Brian L, Floor, Stephen N, Lagna, Giorgio, Hata, Akiko
Format Journal Article
LanguageEnglish
Published United States 23.02.2021
Subjects
Online AccessGet more information

Cover

Loading…
More Information
Summary:Ribosome biogenesis in eukaryotes requires the coordinated production and assembly of 80 ribosomal proteins and four ribosomal RNAs (rRNAs), and its rate must be synchronized with cellular growth. Here, we showed that the Microprocessor complex, which mediates the first step of microRNA processing, potentiated the transcription of ribosomal protein genes by eliminating DNA/RNA hybrids known as R-loops. Nutrient deprivation triggered the nuclear export of Drosha, a key component of the Microprocessor complex, and its subsequent degradation by the E3 ubiquitin ligase Nedd4, thereby reducing ribosomal protein production and protein synthesis. In mouse erythroid progenitors, conditional deletion of led to the reduced production of ribosomal proteins, translational inhibition of the mRNA encoding the erythroid transcription factor Gata1, and impaired erythropoiesis. This phenotype mirrored the clinical presentation of human "ribosomopathies." Thus, the Microprocessor complex plays a pivotal role in synchronizing protein synthesis capacity with cellular growth rate and is a potential drug target for anemias caused by ribosomal insufficiency.
ISSN:1937-9145
DOI:10.1126/scisignal.abd2639