Steric Exclusion Chromatography for Purification of Biomolecules—A Review

Steric exclusion chromatography (SXC) is a purification method that is based on steric exclusion effects from the surface of the target and a hydrophilic stationary phase after the addition of polyethylene glycol (PEG), which leads to an association of the target with the stationary phase without di...

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Bibliographic Details
Published inSeparations Vol. 10; no. 3; p. 183
Main Authors Labisch, Jennifer J., Wiese, G. Philip, Pflanz, Karl
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.03.2023
Subjects
Biochemistry
Biomolecules
Biotechnology
Chromatography
downstream processing
Flow velocity
Hyaluronic acid
Hydrophilicity
Hydrophobicity
Molecular weight
Polyethylene glycol
Polymers
Polyols
Porous materials
Process parameters
Proteins
Purification
steric exclusion chromatography
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Summary:Steric exclusion chromatography (SXC) is a purification method that is based on steric exclusion effects from the surface of the target and a hydrophilic stationary phase after the addition of polyethylene glycol (PEG), which leads to an association of the target with the stationary phase without direct binding, such as covalent, electrostatic, and hydrophilic/hydrophobic interactions. The gentle nature of the method has led to an increased focus on sensitive targets such as enveloped viruses with potential for other sensitive entities, e.g., extracellular vesicles and virus-like particles. SXC is related to PEG-mediated protein precipitation, but investigation of further process parameters was crucial to gain a better understanding of the SXC method. After explaining mechanistic fundamentals and their discovery, this review summarizes the findings on SXC from its first reference 11 years ago until today. Different applications of SXC are presented, demonstrating that the method can be used for a wide variety of targets and achieves high recovery rates and impurity removal. Further, critical process parameters for successful process implementation are discussed, including technical requirements, buffer composition, and scalability.
ISSN:2297-8739
2297-8739
DOI:10.3390/separations10030183