Applications of FT-IR spectrometry to plasma contents analysis and monitoring

• Published in: Vibrational spectroscopy Vol. 32; no. 1; pp. 129 - 136
• Main Authors: Déléris, Gérard, Petibois, Cyril
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 05.08.2003
• Subjects: Classification; Clinics; FT-IR spectrometry; Method; Microsample; Molecular analysis; Plasma; Proteins; FT-IR spectrometry; Proteins; Plasma; Clinics; Classification; Molecular analysis; Method; Microsample
• ISSN: 0924-2031; 1873-3697
• DOI: 10.1016/S0924-2031(03)00053-5

Here, we review recent methodological developments for plasma analysis by Fourier-transform infrared (FT-IR) spectrometry to provide both a high sensitivity and a global overview of its biomolecular contents along with the variations of these ones. Transmittance FT-IR spectrometry has been used to analyze plasma micro samples (50 μl) using an iterative process. Results in accordance with clinical data were obtained from a single FT-IR spectrum for the following biomolecules: amino acids, fatty acids, albumin, glucose, fibrinogen, lactate, triglycerides, glycerol, urea, α 1-antitrypsin, α 2-macroglobulin, transferin, Apo-A 1, Apo-B, Apo-C 3, IgA, IgD, IgG 1, IgG 2, IgG 3, IgG 4, IgM, haptoglobin, α 1-acid glycoprotein, cholesterol, and cholesterol esters. Therefore, as only micro samples are necessary, high frequency blood analysis become available. We also present a novel application of this method for the monitoring of inflammatory processes related to given metabolic stresses in rugby players. We show that an FT-IR spectrum constitutes a “metabolic photography” of the subject, allowing classification between metabolic groups (pathologic or others). It was used on difference spectra in order to raise “signal-to-noise” ratio by elimination of the unvarying spectral contribution. Among others, it allowed to uncover overtraining in high-level sportsmen several weeks before any physiologic or clinical symptom occurred.