Heme oxygenase-1 induction by nitric oxide in RAW 264.7 macrophages is upregulated by a cyclo-oxygenase-2 inhibitor

• Published in: Biochimica et biophysica acta Vol. 1526; no. 1; pp. 13 - 16
• Main Authors: Alcaraz, Maria José, Habib, Aïda, Créminon, Christophe, Vicente, Ana Marı́a, Lebret, Marilyne, Lévy-Toledano, Sylviane, Maclouf, Jacques
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 03.04.2001
• Subjects: Animals; Cell Line; Cyclooxygenase 2; Cyclooxygenase 2 Inhibitors; Cyclooxygenase Inhibitors - pharmacology; Drug Synergism; Heme oxygenase; Heme Oxygenase (Decyclizing) - biosynthesis; Heme Oxygenase-1; Isoenzymes - antagonists & inhibitors; Isoenzymes - biosynthesis; Macrophage; Macrophages - drug effects; Macrophages - enzymology; Membrane Proteins; Mice; Nitric oxide; Nitric Oxide - pharmacology; Nitrogen Oxides; Non-steroidal anti-inflammatory drug; Prostaglandin-Endoperoxide Synthases - biosynthesis; Pyrazoles - pharmacology; RNA, Messenger - biosynthesis; Spermine - analogs & derivatives; Up-Regulation; Nitric oxide; Non-steroidal anti-inflammatory drug; Heme oxygenase; Macrophage
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/S0304-4165(01)00112-X

Unstimulated RAW 264.7 macrophages express negligible heme oxygenase-1 (HO-1) protein but incubation with the nitric oxide (NO) donor spermine nonoate (SPNO) induced HO-1 and weakly cyclo-oxygenase-2 (COX-2) protein. This effect was potentiated by coincubation with the COX-2 selective inhibitor, SC58125. Cells incubated with SPNO showed a strong increase in HO-1 mRNA levels after 4 h with a significant potentiation in the presence of SC58125, which did not modify HO-1 mRNA stability. The induction of HO-1 by NO and its potentiation by anti-inflammatory agents may play a role in inflammatory and immune responses.