Reduction of the minimal sequence for initiation of DNA synthesis by qualitative or quantitative changes of an initiator protein

Initiation of DNA synthesis at an origin of DNA replication involves complex protein-DNA interactions that are still poorly understood. Some of these interactions are highly specific and involve proteins (initiator proteins) thought to be essential for regulation of the initiation process because of...

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Bibliographic Details
Published inNature (London) Vol. 311; no. 5983; p. 279
Main Authors Dotto, G P, Zinder, N D
Format Journal Article
LanguageEnglish
Published England 01.09.1984
Subjects
Bacteriophages - genetics
Base Sequence
DNA Replication
DNA-Binding Proteins - physiology
Viral Proteins - metabolism
Virus Replication
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Summary:Initiation of DNA synthesis at an origin of DNA replication involves complex protein-DNA interactions that are still poorly understood. Some of these interactions are highly specific and involve proteins (initiator proteins) thought to be essential for regulation of the initiation process because of their rate-limiting activity. We show here that both qualitative and quantitative changes in one of these proteins have profound effects on protein-DNA interactions at an origin of DNA replication, and are sufficient to reduce to less than one-third the minimal sequence required for initiation. The general implications of these findings are discussed.
ISSN:0028-0836
DOI:10.1038/311279a0