Shewanella marina sp. nov., isolated from seawater

• Published in: International journal of systematic and evolutionary microbiology Vol. 59; no. 8; pp. 1888 - 1894
• Main Authors: Park, Seong Chan, Baik, Keun Sik, Kim, Mi Sun, Kim, Duwoon, Seong, Chi Nam
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.08.2009; Society for General Microbiology
• Subjects: Anaerobiosis; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; Catalase - metabolism; Cluster Analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Haliotis; Korea; Locomotion; Marine; Microbiology; Molecular Sequence Data; Oxidoreductases - metabolism; Phylogeny; Pigments, Biological - biosynthesis; RNA, Ribosomal, 16S - genetics; Seawater - microbiology; Sequence Analysis, DNA; Shewanella; Shewanella - classification; Shewanella - genetics; Shewanella - isolation & purification; Shewanella - physiology; Systematics; Korea; INW, Korea, Rep; Bacteria; Vibrionaceae; New species; Shewanella; Taxonomy; Seawater
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.005470-0

1 Department of Biology, Sunchon National University, Suncheon 540-742, Republic of Korea 2 Division of Food Science and Aqualife Medicine, Chonnam National University, Yeosu 550-749, Republic of Korea Correspondence Chi Nam Seong scnu{at}scnu.ac.kr A motile, rod-shaped, pale-brown-pigmented bacterium, designated strain C4 T , was isolated from seawater collected from the South Sea (Republic of Korea). Cells were Gram-negative, facultatively anaerobic, and catalase- and oxidase-positive. The major fatty acids were summed feature 3 (C 16 : 1 7 c and/or iso-C 15 : 0 2-OH; 19.4 %), C 16 : 0 (16.3 %), C 17 : 1 8 c (9.5 %) and iso-C 15 : 0 (7.7 %). The DNA G+C content was 40.8 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain C4 T formed a lineage within the genus Shewanella (92.7–96.1 % sequence similarity to representative strains of the genus Shewanella ) and was part of a distinct branch with the clade comprising Shewanella haliotis DW01 T and Shewanella algae ATCC 51192 T . Phenotypic characteristics enabled strain C4 T to be distinguished from S. haliotis and S. algae . On the basis of the data presented in this study, strain C4 T represents a novel species, for which the name Shewanella marina sp. nov. is proposed. The type strain is C4 T (=KCTC 22185 T =JCM 15074 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain C4 T is EU290154 . A scanning electron micrograph and GN2 MicroPlate (Biolog) data for strain C4 T are available as supplementary material with the online version of this paper.