Efficient Purification of 2′-Fucosyllactose by Membrane Filtration and Activated Carbon Adsorption

With the rapid development of synthetic biology, the production of 2′-fucosyllactose by biological fermentation gradually has the basis for industrialization. However, the lack of efficient downstream technology of biological fermentation, especially purification technology, has become the main fact...

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Bibliographic Details
Published inFermentation (Basel) Vol. 8; no. 11; p. 655
Main Authors Wang, Yuanyifei, Hu, Jiaqiang, Sun, Mengyu, Peng, Bo, Wang, Shuo
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.11.2022
Subjects
2′-fucosyllactose
Activated carbon
activated carbon adsorption
Adsorption
Bacteria
Bioengineering
Breastfeeding & lactation
Carbon, Activated
Cellulose acetate
Fermentation
Flocculation
Glucose
Impurities
Lactose
Membrane filtration
Membrane separation
Milk
Oral administration
Prebiotics
Properties
Purification
purification preparation
United States
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Summary:With the rapid development of synthetic biology, the production of 2′-fucosyllactose by biological fermentation gradually has the basis for industrialization. However, the lack of efficient downstream technology of biological fermentation, especially purification technology, has become the main factor limiting its commercialization. In this study, based on the general E. coli biosynthesis of 2′-fucosyllactose fermentation broth, most of the impurities were removed and concentrated using membrane filtration technology after simple flocculation. The target 2′-fucosyllactose was eluted in a targeted manner using activated carbon adsorption and ethanol gradient elution technology. The 2′-fucosyllactose product with 90% or even higher purity could be prepared efficiently. This study explored a new direction for the industrial production of 2′-fucosyllactose.
ISSN:2311-5637
2311-5637
DOI:10.3390/fermentation8110655