Isolation of Crb1, a mouse homologue of Drosophila crumbs, and analysis of its expression pattern in eye and brain

• Published in: Mechanisms of development Vol. 110; no. 1; pp. 203 - 207
• Main Authors: den Hollander, Anneke I., Ghiani, Michela, de Kok, Yvette J.M., Wijnholds, Jan, Ballabio, Andrea, Cremers, Frans P.M., Broccoli, Vania
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 2002
• Subjects: Amino Acid Sequence; Animals; Apico-basal polarity; Bipolar neurons; Brain; Brain - embryology; Brain - growth & development; Central nervous system; Crumbs; Crumbs homologue 1; Dentate gyrus; Drosophila - genetics; Drosophila Proteins - genetics; Epithelial polarity; Eye; Eye - embryology; Eye - growth & development; Eye Proteins; Gene Expression Regulation, Developmental; Genes, Insect; Homeobox Protein Nkx-2.2; Homeodomain Proteins; Humans; Hypothalamus mammillary bodies; In Situ Hybridization; Inner nuclear layer; Iris; Leber congenital amaurosis; Membrane Proteins - genetics; Mice; Molecular Sequence Data; Nerve Tissue Proteins - genetics; Neural stem cell; Neural tube; Nkx2.2; Nuclear Proteins; Olfactory bulbs; Outer nuclear layer; Photoreceptor; Retina; Retinal progenitor; Retinitis pigmentosa; Retinoblasts; Rostral migratory stream; Sequence Homology, Amino Acid; Sonic hedge hog; Subventricular region; Transcription Factors; V3 interneurons; Zona limitans intrathalamica; Brain; Rostral migratory stream; Retinitis pigmentosa; V3 interneurons; Photoreceptor; Central nervous system; Retinal progenitor; Subventricular region; Retina; Bipolar neurons; Apico-basal polarity; Eye; Iris; Neural tube; Inner nuclear layer; Leber congenital amaurosis; Hypothalamus mammillary bodies; Crumbs homologue 1; Outer nuclear layer; Neural stem cell; Crumbs; Olfactory bulbs; Retinoblasts; Nkx2.2; Zona limitans intrathalamica; Dentate gyrus; Sonic hedge hog; Epithelial polarity
• ISSN: 0925-4773; 1872-6356
• DOI: 10.1016/S0925-4773(01)00568-8

Mutations in the human Crumbs homologue 1 ( CRB1) gene cause severe retinal dystrophies. CRB1 is homologous to Drosophila Crumbs, a protein essential for establishing and maintaining epithelial polarity. We have isolated the mouse orthologue, Crb1, and analyzed its expression pattern in embryonic and post-natal stages. Crb1 is expressed exclusively in the eye, and the central nervous system. In the developing eye, expression of Crb1 is detected in the retinal progenitors, and later on becomes restricted to the differentiated photoreceptor cells where it remains active up to the adult stage. In the developing neural tube, expression of Crb1 is restricted to its most ventral structures, coinciding with the expression domain of Nkx2.2. In the adult brain, Crb1 expression is defined to areas where the production and migration of neurons occurs in adulthood.