Using Ruditapes philippinarum conglutination mud to produce bioflocculant and its applications in wastewater treatment

• Published in: Bioresource technology Vol. 100; no. 21; pp. 4996 - 5001
• Main Authors: Gao, Qi, Zhu, Xiu-Hua, Mu, Jun, Zhang, Yi, Dong, Xue-Wei
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.11.2009; Elsevier
• Subjects: Animals; Applied sciences; Bacteria - cytology; Bacteria - isolation & purification; Biodegradation, Environmental; Bioflocculant; Biological and medical sciences; Bivalvia - microbiology; Color; Coloring Agents - chemistry; Conglutination mud; Exact sciences and technology; Flocculation; Fundamental and applied biological sciences. Psychology; Fungi - cytology; Hydrogen-Ion Concentration; Ions; Kaolin - chemistry; Metals, Heavy - isolation & purification; Pollution; Polymerase Chain Reaction; Ruditapes philippinarum; Sewage - microbiology; Soil Microbiology; Solutions; Temperature; Waste Disposal, Fluid; Wastewater treatment; Wastewaters; Water Purification; Water treatment and pollution; Bioflocculant; Conglutination mud; Wastewater treatment; Ruditapes philippinarum; Flocculation reagent; Waste water purification; Mud
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2009.05.035

A novel bioflocculant-producing bacterium, ZHT4-13, was isolated from Ruditapes philippinarum conglutination mud. By biomicroscope morphological observation, 16S rDNA sequence identification and physiological and biochemical characteristics, strain ZHT4-13 was identified as Rothia sp. The bioflocculant MBF4-13 produced by strain ZHT4-13 had a flocculating efficiency of 86.22% for 5 g L −1 Kaolin clay suspension when the initial pH was 9 and the temperature was 20 °C. It had flocculating effect in a wide range, pH 1–13 and temperature 4–100 °C. Analysis of MBF4-13 by UV–Vis spectrophotometer, Fourier-transform infrared spectrophotometer (FT-IR) and 1H nuclear magnetic resonance (NMR) indicated that the main component of MBF4-13 is polysaccharide. The culture conditions to produce strain ZHT4-13 were optimized with orthogonal design of experiments. MBF4-13 had high efficiency in decolorizing dye solutions, had some abilities to remove heavy metal ions ( Cr 2 O 7 2 − , Ni 2+) and improve performance of activated sludge.