Densitometric high performance thin-layer chromatography identification and quantitative analysis of psychotropic drugs

• Published in: Journal of AOAC International Vol. 88; no. 1; p. 70
• Main Authors: Maślanka, Anna, Krzek, Jan
• Format: Journal Article
• Language: English
• Published: England 01.01.2005
• Subjects: Amitriptyline - analysis; Antipsychotic Agents - analysis; Chemistry Techniques, Analytical - methods; Chlorpromazine - analysis; Chromatography; Chromatography, High Pressure Liquid - methods; Chromatography, Thin Layer - methods; Clonazepam - analysis; Densitometry - methods; Diazepam - analysis; Doxepin - analysis; Fluphenazine - analysis; Haloperidol - analysis; Pharmaceutical Preparations - analysis; Promazine - analysis; Psychotropic Drugs - analysis; Regression Analysis; Sulpiride - analysis; Trifluoperazine - analysis; Ultraviolet Rays
• ISSN: 1060-3271
• DOI: 10.1093/jaoac/88.1.70

A thin-layer chromatography (TLC)-densitometry method has been developed to identify and quantify haloperidol, amitriptyline, sulpiride, promazine, fluphenazine, doxepin, diazepam, trifluoperazine, clonazepam, and chlorpromazine in selected psychotropic drugs. Separation was performed on precoated silica gel 60 F254 TLC plates. Chromatograms were developed in various mobile phases, and 8 of 30 tested phases were selected based on spot location and developing time. The identification and quantification were carried out based on ultraviolet densitometric measurements at chosen wavelengths. In addition to retention coefficients, the absorption spectra recorded directly from chromatograms were also used in qualitative analysis. Under established experimental conditions, high sensitivity of the method was achieved. The limit of detection ranged from 0.009 to 0.260 microg, depending on the wavelength selected for measuring. A satisfactory recovery, ranging from 92.99 to 104.70%, was achieved for individual constituents.