Do trichothecenes reduce viability of circulating blood cells and modify haemostasis parameters?

• Published in: Mycopathologia (1975) Vol. 156; no. 4; pp. 349 - 356
• Main Authors: Froquet, R, Arnold, F, Batina, P, Parent-Massin, D
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.01.2003; Springer Nature B.V
• Subjects: Anemia; Biological and medical sciences; Blood; blood coagulation; Blood Glucose - metabolism; Blood platelets; Bone marrow; cell aggregates; cytotoxicity; deoxynivalenol; erythrocytes; Erythrocytes - drug effects; Fetal Blood - metabolism; Fundamental and applied biological sciences. Psychology; glucose; glutathione; Glutathione - blood; Hematology; hematopoiesis; Hematopoiesis - drug effects; Hemoglobins - metabolism; hemolysis; Hemorrhage; Humans; Infectious diseases; lactic acid; Lactic Acid - blood; Leukocyte Count; Leukocytes; Leukocytes - drug effects; Leukopenia; Medical sciences; Microbiology; Mycology; Mycoses; Partial Thromboplastin Time; Pathogenicity, host-agent relations, miscellaneous strains, epidemiology; Platelet Aggregation - drug effects; Platelet Aggregation - physiology; Prothrombin Time; Solvents; T-2 toxin; T-2 Toxin - pharmacology; Thrombin Time; Thrombocytopenia; trichothecenes; Trichothecenes - pharmacology; France; Toxin; Blood cell; DON; erythrocytes; Microbiology; Mycology; leucocytes; Red blood cell; T-2 toxin; trichothecenes; Coagulation parameters; haematopoiesis
• ISSN: 0301-486X; 1573-0832
• DOI: 10.1023/B:MYCO.0000003606.13934.74

This manuscript describes the results of experiments conducted using human blood cells to determine the ability of T-2 toxin and DON to cause changes in clotting time, platelet aggregation, red blood cell haemolysis, RBC glucose content, lactate release, glutathione depletion, as well as white blood cell viability. In vitro results showed that haemostasis parameters and erythrocytes were not affected at concentrations able to induce inhibition of haematopoietic progenitor proliferation. In the presence of 10(-8) M and 10(-6) M T-2, the leucocyte number decreased at 24 h by 30% and 50% respectively. A 50% decrease in leucocyte number was observed for 10(-5) M DON. Results were compared with haematopoietic progenitor sensitivities. Due to the differences in sensitivities between mature blood cells and haematopoietic progenitors, haematological problems associated with trichothecene intoxication could be attributed to haematopoiesis inhibition.