Kupffer Cells Infiltrate Liver Tissue Early after Ischemia-Reperfusion and Partial Hepatectomy

• Published in: European surgical research Vol. 37; no. 5; pp. 290 - 297
• Main Authors: Baier, P.K., Baumgartner, U., Hempel, S., Wolff-Vorbeck, G., von Dobschuetz, E., Hopt, U.T.
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland Karger 01.09.2005; S. Karger AG
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Biological and medical sciences; General aspects; Hepatectomy; Interleukin-1 - analysis; Ki-67 Antigen - analysis; Kupffer Cells - physiology; Liver - immunology; Liver - pathology; Liver Regeneration - immunology; Male; Medical sciences; Original Paper; Rats; Rats, Sprague-Dawley; Reperfusion Injury - immunology; Tumor Necrosis Factor-alpha - analysis; Ischemia-reperfusion; Ki-67; ED2; Ischemia; Kupffer cells; Sepsis; Partial hepatectomy; IL-1β; Liver regeneration; Liver; Hepatectomy; Sepsis syndrome; Tissue; Infiltrate; Partial; Surgery; Partial hepatectomy, Kupffer cells; Anesthesia; Resuscitation; ED2+, Ischemia; Ischemia reperfusion; Interleukin 1β; Bacteremia; Infection; Medicine; Kupffer cell; Regeneration; Treatment; Bacteriosis; Ki67 antigen; Early
• ISSN: 0014-312X; 1421-9921
• DOI: 10.1159/000089239

Kupffer cells, ED2+ macrophages of the liver, play an important role in liver damage and regeneration. It is proposed that Kupffer cells are stationary and regenerate after acute liver trauma by local proliferation. We analyzed their kinetics in three surgically relevant murine models of acute liver injury: partial liver resection, ischemia with reperfusion and sepsis. We found an early increase in ED2+ cells after 0.5 h and a maximum after 12 h. These results suggest an infiltration of the cells early after the injury and a later local proliferation. These ED2+ macrophages are localized predominantly periportally; nearly no macrophages are found pericentrally, except in the sepsis model. Therefore, a shifting of macrophages from portal to central seems to be unlikely, suggesting a hepatic zonation of homing factors.