Study of the influence of derivatization conditions on the structure and stability of nucleoside derivatives using gas chromatography-mass spectrometry
Conditions of the derivatization of nucleosides are studied using various reagents. Single trimethylsilyl derivatives of uridine, 5-methyluridine, cytidine, 5-methylcytidine, inosine, xanthosine, N 2 , N 2 -dimethylguanosine were obtained by reaction with N , O -bis(trimethylsilyl)trifluoroacetamide...
Saved in:
Published in | Journal of analytical chemistry (New York, N.Y.) Vol. 69; no. 14; pp. 1307 - 1312 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Moscow
Pleiades Publishing
30.12.2014
Springer |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Conditions of the derivatization of nucleosides are studied using various reagents. Single trimethylsilyl derivatives of uridine, 5-methyluridine, cytidine, 5-methylcytidine, inosine, xanthosine,
N
2
,
N
2
-dimethylguanosine were obtained by reaction with
N
,
O
-bis(trimethylsilyl)trifluoroacetamide. A mixture of trimethylsilyl derivatives of adenosine and guanosine formed under these conditions.
N
-trifluoroacetyl-
O
-trimethylsilyl derivatives of cytidine, 5-methylcytidine, adenosine (in mixture with trimethylsilyl derivative) were prepared using
N
,
O
-bis(trimethylsilyl)trifluoroacetamide and
N
-methyl-bis(trifluoroacetamide) (mixed derivatization). Derivatives of cytidine and 5-methylcytidine obtained by mixed derivatization possessed better chromatographic characteristics compared to trimethylsilyl derivatives. The formation of a trifluoroacetyl derivatives of adenosine by the action of
N
-methyl-bis(trifluoroacetamide) is shown. The possibility of the replacement of the reagent and pyridine by an inert solvent is studied. |
---|---|
ISSN: | 1061-9348 1608-3199 |
DOI: | 10.1134/S106193481414007X |