Structure and stress-related expression of two cDNAs encoding proteinase inhibitor II of Nicotiana glutinosa L

• Published in: Biochimica et biophysica acta Vol. 1492; no. 1; pp. 211 - 215
• Main Authors: Choi, Doil, Park, Jong-A, Seo, Young Sam, Chun, Yoon Joo, Kim, Woo Taek
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 21.06.2000
• Subjects: Amino Acid Sequence; cDNA; DNA, Complementary - analysis; DNA, Plant - analysis; Molecular Sequence Data; Nicotiana - genetics; Nicotiana - virology; Nicotiana glutinosa L; Plant Proteins - biosynthesis; Plant Proteins - genetics; Plants, Toxic; Protease Inhibitors; Proteinase inhibitor; Restriction Mapping; Sequence Homology, Amino Acid; Stress-induced gene expression; Tissue-specific expression; Tobacco Mosaic Virus; cDNA; Tissue-specific expression; Nicotiana glutinosa L; Stress-induced gene expression; Tobacco mosaic virus; Proteinase inhibitor
• ISSN: 0167-4781; 0006-3002; 1879-2634; 1878-2434
• DOI: 10.1016/S0167-4781(00)00073-7

Two cDNAs, pNGPI-1 and pNGPI-2, encoding Nicotiana glutinosa proteinase inhibitor II (PI-II) have been cloned, sequenced and identified. The deduced amino acid sequences are 54–82% identical to those of other plant PI-II. The NGPI-1 protein is composed of eight repeated domains, while NGPI-2 contains six repeated regions, each with a putative reactive site. The expression of NGPI-1 is highly regulated in a developmental- and tissue-specific manner, with the transcript being detected in young leaves and floral organs of N. glutinosa plants. In mature leaves, the NGPI-1 gene is rapidly activated by distinct temporal induction patterns in response to pathogen-related (biotic) and wound-related (abiotic) stresses.