The phytoestrogen equol increases nitric oxide availability by inhibiting superoxide production: an antioxidant mechanism for cell-mediated LDL modification

• Published in: Free radical biology & medicine Vol. 34; no. 10; pp. 1271 - 1282
• Main Authors: Hwang, Juliana, Wang, Jian, Morazzoni, Paolo, Hodis, Howard N., Sevanian, Alex
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.05.2003
• Subjects: Animals; Antioxidants - pharmacology; Biological Availability; Cells, Cultured; Chromans - pharmacology; Copper - metabolism; Electronegative LDL; Equol; Estrogens, Non-Steroidal - pharmacology; Free radicals; Genistein - pharmacology; Iron - metabolism; Isoflavones; Isoflavones - pharmacology; Kinetics; Lipid Peroxidation; Lipoproteins, LDL - metabolism; Macrophages - drug effects; Macrophages - metabolism; Mice; Monocytes - drug effects; Monocytes - metabolism; NADPH oxidase; NADPH Oxidases - metabolism; Nitric oxide; Nitric Oxide - metabolism; Nitric oxide synthase; Oxidation-Reduction; Oxidized LDL; Phytoestrogens; Plant Preparations; Superoxides - antagonists & inhibitors; Superoxides - metabolism; Time Factors; Free radicals; Electronegative LDL; Oxidized LDL; NADPH oxidase; Nitric oxide; Nitric oxide synthase; Isoflavones
• ISSN: 0891-5849; 1873-4596
• DOI: 10.1016/S0891-5849(03)00104-7

Estrogen replacement therapy (ERT) is reported to lower the incidence of cardiovascular disease in postmenopausal women. ERT also lowers the levels of oxidatively modified low-density lipoprotein (LDL). Because modified LDL can mediate the development of atherosclerosis by inflammatory processes, ERT may exert its LDL protective effect through enhanced antioxidant activity in vascular tissues. Plant sources of estrogenic compounds have been used as alternatives for ERT because they avoid a number of negative health effects produced by estrogen. In this study, the antioxidant properties of the soy isoflavone metabolite, equol (an estrogenic metabolite of daidzein) were studied. Equol has a greater antioxidant activity than the parent isoflavone compounds genistein and daidzein, found in high concentration in soy. Equol inhibits LDL oxidation in vitro and LDL oxidative modification by J774 monocyte/macrophages to LDL −, an electronegative modified LDL found in human plasma. An antioxidant effect of equol was found to be mediated by inhibition of superoxide radical (O 2 −•) production and manifested through enhanced levels of free nitric oxide (NO) that prevents LDL modification. Thus, when NO levels were increased by donor agents, generators, or compounds that facilitate nitric oxide synthase activity, LDL − formation by J774 cells was strongly inhibited. Conversely, inhibition of NO production enhanced LDL − formation, and the combination of reduced NO and increased O 2 −• production yielded maximum LDL − formation. Pretreatment of cells with equol inhibited production of O 2 −• by J774 cells apparently via the inactivation of the reduced nicotinamide adenine dinucleotide phosphate oxidase complex. Decreased O 2 −• production resulted in increased free NO levels (but not total NO production) indicating that decreased reactions between O 2 −• and NO are an outcome of equol’s antioxidant activity in cell culture.