Proliferating cell nuclear antigen and Ki67 immunoreactivity in ameloblastomas

• Published in: Oral surgery, oral medicine, oral pathology, oral radiology and endodontics Vol. 95; no. 2; pp. 213 - 221
• Main Authors: Meer, Shabnum, Galpin, Jacqueline S., Altini, Mario, Coleman, Hedley, Ali, Hasiena
• Format: Journal Article
• Language: English
• Published: St. Louis, MO Mosby, Inc 01.02.2003; Elsevier
• Subjects: Ameloblastoma - chemistry; Ameloblastoma - pathology; Analysis of Variance; Biological and medical sciences; Cell Division; Dentistry; Ent. Stomatology; Facial bones, jaws, teeth, parodontium: diseases, semeiology; Humans; Immunohistochemistry; Investigative techniques, diagnostic techniques (general aspects); Jaw Neoplasms - chemistry; Jaw Neoplasms - pathology; Ki-67 Antigen - analysis; Medical sciences; Observer Variation; Otorhinolaryngology. Stomatology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Proliferating Cell Nuclear Antigen - analysis; Tumors; Human; Immunohistochemistry; Prognosis; Stomatology; Diseases of the osteoarticular system; Clinical form; Activity; Exploration; Malignant tumor; Proliferation; Pathology; Adamantinoma; Odontogenic tumor; Biopsy; Histological type; Ki67 antigen; PCNA antigen; Comparative study; Maxillary disease
• ISSN: 1079-2104; 1528-395X
• DOI: 10.1067/moe.2003.62

Objective. This study used proliferating cell nuclear antigen (PCNA) and Ki67 to evaluate and compare the in situ proliferative activity of (1) solid and multicystic and (2) unicystic ameloblastomas in an attempt to provide a scientific basis for any differences in biologic behavior that exists between these 2 groups of lesions. Study design. Twenty archival tissue sections, 10 of primary unicystic and 10 of solid and multicystic ameloblastomas, were immunohistochemically stained with PCNA and Ki67 antisera. Immunoreactivity was evaluated by the counting of cells, and the data obtained were statistically analyzed. Results. The results showed that cellular proliferative activity varied within the ameloblastoma types. The unicystic ameloblastomas showed statistically significantly higher PCNA and Ki67 labeling indices than the solid and multicystic variant. Conclusion. There appears, therefore, to be no correlation between proliferative activity as shown by these proteins and reported biologic behavior. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003;95:213-21)