Cobalt(II) salophen-modified carbon-paste electrode for potentiometric and voltammetric determination of cysteine

• Published in: Analytical biochemistry Vol. 320; no. 1; pp. 32 - 38
• Main Authors: Amini, Mohammad K., Khorasani, Jafar H., Khaloo, Shokooh S., Tangestaninejad, Shahram
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2003
• Subjects: Carbon - chemistry; Carbon-paste electrode; Cobalt - chemistry; Cobalt(II) salophen; Cysteine; Cysteine - analysis; Electric Conductivity; Electrochemistry - instrumentation; Electrodes; Modified electrode; Potentiometric sensor; Potentiometry - instrumentation; Salicylates - chemistry; Sensors; Carbon-paste electrode; Cysteine; Potentiometric sensor; Cobalt(II) salophen; Sensors; Modified electrode
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/S0003-2697(03)00355-5

A chemically modified electrode constructed by incorporating N, N ′-bis(salicylidene)-1,2-phenylenediaminocobalt(II) into carbon-paste matrix was used as a sensitive electrochemical sensor for detection of cysteine. The resulting electrode exhibits catalytic properties for the electrooxidation of cysteine and lowers the overpotential for the oxidation of this compound. The faster rate of electron transfer results in a near-Nernstian behavior of the modified electrode and makes it a suitable potentiometric and voltammetric sensor for the fast and easy determination of cysteine. A linear response in concentration range from ∼2 μM to 0.01 M was obtained with a detection limit of 1 μM for the potentiometric detection of cysteine. The modified electrode was also used for the amperometric and differential pulse voltammetric determination of cysteine and the results were compared with those of the potentiometric method.