Hemoglobin-sulfhydryls from tortoise ( Geochelone carbonaria) can reduce oxidative damage induced by organic hydroperoxide in erythrocyte membrane

Sulfhydryl groups are important to avoid oxidative damage to the cell. In RBC, tert-butyl hydroperoxide ( tert-BOOH) and hydrogen peroxide (H 2O 2) are capable of oxidizing heme and promoting lipid peroxidation. H 2O 2 caused greater oxidation of heme than tert-BOOH, although the oxidation of sulfhy...

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Published inComparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Vol. 126; no. 4; pp. 571 - 577
Main Authors Torsoni, M.A, Ogo, S.H
Format Journal Article
LanguageEnglish
Published England Elsevier Inc 01.08.2000
Subjects
Animals
Erythrocyte
Erythrocyte Membrane - drug effects
Erythrocyte Membrane - metabolism
Free radicals
Hemichrome
Hemoglobin
Hemoglobins - antagonists & inhibitors
Hemoglobins - chemistry
Hemoglobins - metabolism
Hemoglobins - pharmacology
Humans
Hydrogen Peroxide - pharmacology
Lipid peroxidation
Lipid Peroxidation - drug effects
Membrane
Organic hydroperoxide
Oxidation-Reduction
Sulfhydryl Compounds - antagonists & inhibitors
Sulfhydryl Compounds - chemistry
Sulfhydryl Compounds - metabolism
Sulfhydryl group
tert-Butylhydroperoxide - pharmacology
Thiobarbituric Acid Reactive Substances - metabolism
Tortoise
Turtles - blood
Erythrocyte
Free radicals
Organic hydroperoxide
Hemichrome
Hemoglobin
Lipid peroxidation
Membrane
Tortoise
Sulfhydryl group
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Summary:Sulfhydryl groups are important to avoid oxidative damage to the cell. In RBC, tert-butyl hydroperoxide ( tert-BOOH) and hydrogen peroxide (H 2O 2) are capable of oxidizing heme and promoting lipid peroxidation. H 2O 2 caused greater oxidation of heme than tert-BOOH, although the oxidation of sulfhydryl groups was similar. Geochelone carbonaria Hb, a rich sulfhydryl protein, inhibited the TBA-reactive substances formation of human erythrocytes exposed to tert-BOOH by about 30%; this decrease was smaller with Geochelone denticulata Hb. Sulfhydryl reagents diminished the number of reactive sulfhydryl groups in the G. carbonaria Hb resulting in a decrease of its antioxidant power, suggesting the involvement of sulfhydryls of Hb in the protection against lipid peroxidation.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1096-4959
1879-1107
DOI:10.1016/S0305-0491(00)00230-3