Cloning and mapping of the CYS4 gene of Saccharomyces cerevisiae

A DNA fragment containing the CYS4 gene of Saccharomyces cerevisiae was isolated from a genomic library. The cloned fragment hybridized to the transverse-alternating-field-electrophoresis band corresponding to chromosomes VII and XV. According to the 2 microns DNA chromosome-loss procedure, the cys2...

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Bibliographic Details
Published inCurrent genetics Vol. 21; no. 4-5; p. 285
Main Authors Ono, B.I. (Okayama Univ. (Japan). Faculty of Pharmaceutical Sciences. Lab. of Environmental Hygiene Chemistry), Heike, C, Yano, Y, Inoue, T, Naito, K, Nakagami, S, Yamane, A
Format Journal Article
LanguageEnglish
Published United States 01.04.1992
Subjects
Acetyltransferases - genetics
BIOSINTESIS
BIOSYNTHESE
BIOSYNTHESIS
CARTE GENETIQUE
Chromosome Mapping
CISTEINA
CLONACION MOLECULAR
CLONAGE MOLECULAIRE
Cloning, Molecular
CYS4
Cystathionine beta-Synthase - genetics
CYSTEINE
Cysteine - biosynthesis
Cysteine biosynthetic
GENE
GENES
Genes, Fungal
GENETIC MAPS
MAPAS GENETICOS
Mapping
MOLECULAR CLONING
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Serine O-Acetyltransferase
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Summary:A DNA fragment containing the CYS4 gene of Saccharomyces cerevisiae was isolated from a genomic library. The cloned fragment hybridized to the transverse-alternating-field-electrophoresis band corresponding to chromosomes VII and XV. According to the 2 microns DNA chromosome-loss procedure, the cys2 and cys4 mutations, which are linked together and co-operatively confer cysteine dependence, were assigned to chromosome VII. By further mapping involving tetrad analysis, the cys2-cys4 pair was localized between SUP77 (SUP166) and ade3 on the right arm of chromosome VII.
Bibliography:93B0457
F30
ISSN:0172-8083
1432-0983
DOI:10.1007/bf00351684