High-Performance Liquid Chromatographic Separation of Metal Ions with 2-Hydroxy-5-sulfoaniline-N-salicylidene

• Published in: Analytical Sciences Vol. 10; no. 6; pp. 947 - 949
• Main Authors: ZENKI, Michio, INOUE, Kazue
• Format: Journal Article
• Language: English
• Published: Tokyo The Japan Society for Analytical Chemistry 01.12.1994; Japan Society for Analytical Chemistry; Japan Science and Technology Agency
• Subjects: 2-hydroxy-5-sulfoaniline-N-salicylidene; Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; High-performance liquid chromatography; Other chromatographic methods; precolumn complexation; spectrophotometric detection; Separation; Precolumn; HPLC chromatography; Organic ligand; Metal Ions; Metal complex; Phenols; Imine; Derivatization
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.10.947

High-performance liquid chromatography (HPLC) for the separation and determination of metal ions as their chelates with organic reagents has received increasing attention in recent years. An attractive approach is the precolumn method, in which the metal chelate formation is completed prior to the separation and determination by HPLC. In most cases, a reagent forms less soluble chelates in water, and the chelates and the reagent itself are held strongly on the analytical column. Thus an organic modifier such as methanol, acetonitrile or a surfactant is added to the mobile phase in order to keep the chelates in solution or to elute them from the column. Organic solvents for HPLC purposes are, however, expensive and must be treated after use. Moreover, organic modifiers have different absorbances and refractive indexes from water, and these make baseline noises and troublesome system peaks. The ideal mobile phase for the reversed-phase HPLC should be an aqueous solution. In this paper, a sulfonated derivative of salicylidene omicron -aminophenol, 2-hydroxy-5-sulfoaniline-N-salicylidene (HSS) has been investigated as a precolumn chelating reagent for the reversed-phase HPLC with spectrophotometric detection. Neat aqueous solutions (0.05 M acetate buffer, pH 5.0) have been used in both complexation and chromatographic elution.