High-Performance Liquid Chromatographic Separation of Metal Ions with 2-Hydroxy-5-sulfoaniline-N-salicylidene

High-performance liquid chromatography (HPLC) for the separation and determination of metal ions as their chelates with organic reagents has received increasing attention in recent years. An attractive approach is the precolumn method, in which the metal chelate formation is completed prior to the s...

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Bibliographic Details
Published inAnalytical Sciences Vol. 10; no. 6; pp. 947 - 949
Main Authors ZENKI, Michio, INOUE, Kazue
Format Journal Article
LanguageEnglish
Published Tokyo The Japan Society for Analytical Chemistry 01.12.1994
Japan Society for Analytical Chemistry
Japan Science and Technology Agency
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Summary:High-performance liquid chromatography (HPLC) for the separation and determination of metal ions as their chelates with organic reagents has received increasing attention in recent years. An attractive approach is the precolumn method, in which the metal chelate formation is completed prior to the separation and determination by HPLC. In most cases, a reagent forms less soluble chelates in water, and the chelates and the reagent itself are held strongly on the analytical column. Thus an organic modifier such as methanol, acetonitrile or a surfactant is added to the mobile phase in order to keep the chelates in solution or to elute them from the column. Organic solvents for HPLC purposes are, however, expensive and must be treated after use. Moreover, organic modifiers have different absorbances and refractive indexes from water, and these make baseline noises and troublesome system peaks. The ideal mobile phase for the reversed-phase HPLC should be an aqueous solution. In this paper, a sulfonated derivative of salicylidene omicron -aminophenol, 2-hydroxy-5-sulfoaniline-N-salicylidene (HSS) has been investigated as a precolumn chelating reagent for the reversed-phase HPLC with spectrophotometric detection. Neat aqueous solutions (0.05 M acetate buffer, pH 5.0) have been used in both complexation and chromatographic elution.
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ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.10.947