Sensitive and efficient determination of gabapentin in human serum based on capillary electrophoresis with laser-induced fluorescence detection

• Published in: Journal of analytical chemistry (New York, N.Y.) Vol. 68; no. 7; pp. 639 - 645
• Main Authors: Cao, Li-Wei, Hu, Yang, Liang, Si-Liu, Li, Cong, Meng, Jian-Xin
• Format: Journal Article
• Language: English
• Published: Dordrecht SP MAIK Nauka/Interperiodica 01.07.2013; Springer
• Subjects: Analytical Chemistry; Chemical properties; Chemistry; Chemistry and Materials Science; Composition; Electrophoresis; Fluorescence; Gabapentin; Identification and classification; Methods; Pharmaceutical chemistry; Serum; China; 6-oxy-; laser-induced fluorescence detection; gabapentin; succinimidyl acetate)-9-(2′-methoxycarbonyl) fluorescein; γ-aminobutyric acid; capillary electrophoresis
• ISSN: 1061-9348; 1608-3199
• DOI: 10.1134/S1061934813070071

A sensitive and efficient analytical method for gabapentin ( GBP ) in human serum based on capillary electrophoresis ( CE ) with laser-induced fluorescence ( LIF ) detection has been established. 6-Oxy-( N -succinimidyl acetate)-9-(2′-methoxycarbonyl) fluorescein ( SAMF ), a new synthesized fluorescent reagent, was used for precolumn derivatization of the non-fluorescent drug in serum. γ-Aminobutyric acid ( GABA ) was used as an internal standard ( I.S. ). The best derivative condition was obtained in phosphate buffer (pH 8) at room temperature for 10 min. Optimal separation and detection were obtained with a background electrolyte ( BGE ) of 3.5 × 10 −2 M phosphate buffer (pH 5.5) and laser-induced fluorescence detection excited at 473 nm. The method developed for GBP was linear over the concentration range of 4.0 × 10 −9 to 4.0 × 10-7 M. The concentration limit of detection was 2.0 × 10 −10 M (signal-to-noise ratio = 3). The sensitive method was used for the determination of GBP in serum samples.