Rapid Analysis of the Chemical Compositions in Semiliquidambar cathayensis Roots by Ultra High-Performance Liquid Chromatography and Quadrupole Time-of-Flight Tandem Mass Spectrometry

• Published in: Molecules (Basel, Switzerland) Vol. 24; no. 22; p. 4098
• Main Authors: Yang, Li, Liu, Rong-Hua, He, Jun-Wei
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 13.11.2019; MDPI
• Subjects: Alkaloids; Arthritis; Chemical composition; Chinese medicine; Chromatography; Chromatography, High Pressure Liquid; Fatty acids; Flavonoids; Hamamelidaceae - chemistry; Herbal medicine; High performance liquid chromatography; Ions; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Medicinal plants; Molecular Structure; Peptides; Phenylpropanoids; Phytochemicals - analysis; Phytochemicals - chemistry; Plant Extracts - analysis; Plant Extracts - chemistry; Plant Roots - chemistry; Quadrupoles; Rheumatism; Roots; Scientific imaging; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectroscopy; UHPLC-Q-TOF-MS/MS; chemical profiling; rapid identification; Semiliquidambar cathayensis roots; chemical compositions
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules24224098

Chang was a traditional medicinal plant and used to treat rheumatism arthritis and rheumatic arthritis for centuries in China with no scientific validation, while only 15 components were reported. Thus, a rapid, efficient, and precise method based on ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was applied in both positive- and negative-ion modes to rapidly analysis the main chemical compositions in for the first time. Finally, a total of 85 chemical compositions, including 35 alkaloids, 12 flavonoids, 7 terpenoids, 5 phenylpropanoids, 9 fatty acids, 7 cyclic peptides, and 10 others were identified or tentatively characterized in the roots of based on the accurate mass within 5 ppm error. Moreover, alkaloid, flavonoid, phenylpropanoid, and cyclic peptide were reported from for the first time. This rapid and sensitive method was highly useful to comprehend the chemical compositions and will provide scientific basis for further study on the material basis, mechanism and clinical application of roots.