Single-Molecule Sequencing Assists Genome Assembly Improvement and Structural Variation Inference

The single-molecule real-time （SMRT） sequencing platform pre- sented by Pacific Biosciences （PacBio） is regarded as a third- generation sequencing technology （Eid et al, 2009; Roberts et al., 2013）. PacBio delivers long reads from several to tens of kilobases （kbs）, which are ideal for filling unseq...

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Published inMolecular plant Vol. 9; no. 7; pp. 1085 - 1087
Main Authors Yan, Jun, Lv, Shuwei, Hu, Min, Gao, Zhaoxu, He, Hang, Ma, Qingwei, Deng, Xing Wang, Zhu, Zuofeng, Wang, Xiangfeng
Format Journal Article
LanguageEnglish
Published England Elsevier Inc 06.07.2016
Subjects
GC含量
Genome, Plant - genetics
High-Throughput Nucleotide Sequencing - methods
Sequence Analysis, DNA - methods
单分子
基因组
插入/缺失
测序技术
生物科学
结构变化
装配
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Summary:The single-molecule real-time （SMRT） sequencing platform pre- sented by Pacific Biosciences （PacBio） is regarded as a third- generation sequencing technology （Eid et al, 2009; Roberts et al., 2013）. PacBio delivers long reads from several to tens of kilobases （kbs）, which are ideal for filling unsequenced gaps due to unusual sequence contexts, such as high~GC content or repeat-rich regions （Bashir et al., 2012; Berlin et al., 2015; Chaisson et al., 2015）. PacBio long reads are also favorable for detecting large DNA fragments harboring structural variations （SVs）, such as inversions, translocations, duplications, and large insertions/deletions （indels） （Ritz et al., 2010; English et al., 2014）.
Bibliography:31-2013/Q
The single-molecule real-time （SMRT） sequencing platform pre- sented by Pacific Biosciences （PacBio） is regarded as a third- generation sequencing technology （Eid et al, 2009; Roberts et al., 2013）. PacBio delivers long reads from several to tens of kilobases （kbs）, which are ideal for filling unsequenced gaps due to unusual sequence contexts, such as high~GC content or repeat-rich regions （Bashir et al., 2012; Berlin et al., 2015; Chaisson et al., 2015）. PacBio long reads are also favorable for detecting large DNA fragments harboring structural variations （SVs）, such as inversions, translocations, duplications, and large insertions/deletions （indels） （Ritz et al., 2010; English et al., 2014）.
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content type line 63
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ISSN:1674-2052
1752-9867
DOI:10.1016/j.molp.2016.04.002