Chromatographic methods for the determination of fluphenazine, nortriptyline and its impurity amitriptyline in bulk and pharmaceutical formulations
Two selective, reproducible methods were proposed for the simultaneous determination of Fluphenazine hydrochloride ( Flu ) and Nortriptyline hydrochloride ( Nor ) in the presence of its impurity Amitriptyline hydrochloride ( Ami ). The first method is TLC-densitometric on silica gel with ethyl aceta...
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Published in | Journal of analytical chemistry (New York, N.Y.) Vol. 69; no. 12; pp. 1187 - 1192 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Moscow
Pleiades Publishing
01.12.2014
Springer |
Subjects | |
Online Access | Get full text |
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Summary: | Two selective, reproducible methods were proposed for the simultaneous determination of Fluphenazine hydrochloride (
Flu
) and Nortriptyline hydrochloride (
Nor
) in the presence of its impurity Amitriptyline hydrochloride (
Ami
). The first method is TLC-densitometric on silica gel with ethyl acetate-methanol-concentrated ammonia 2: 7: 0.2 (v/v/v) as mobile phase. The linear range 0.5–20, 0.5–5 and 0.5–5 μg per spot for Nor, Ami and Flu respectively. The second method is HPLC on Genesis C
18
column with 0.1 M ammonium acetate-acetonitrile 1: 1 (v/v) as mobile phase and Ultraviolet detection at 254 nm. The LC method was linear over concentration ranges 100–500, 50–300 and 10–250 μg/mL for Nor, Ami and Flu respectively. The proposed methods were successfully applied for determination of these drugs in laboratory prepared mixtures, and in pharmaceutical dosage forms showing good accuracy and precision. The results obtained agreed statistically with those obtained by using the official methods. |
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ISSN: | 1061-9348 1608-3199 |
DOI: | 10.1134/S1061934814120041 |