Protein phosphorylation in fast and slow chicken skeletal muscles: Effect of denervation
We have identified proteins in adult chicken skeletal muscle whose phosphorylation can be used as markers for the mature fast and slow muscle phenotype. These include phosphorylase, phosphorylase kinase, and a cyclic adenosine 3′,5′‐monophosphate (cAMP)‐stimulated, calmodulin‐inhibited 28‐kDa band (...
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Published in | Muscle & nerve Vol. 21; no. 4; pp. 504 - 513 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
New York
John Wiley & Sons, Inc
01.04.1998
Wiley |
Subjects | |
Online Access | Get full text |
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Summary: | We have identified proteins in adult chicken skeletal muscle whose phosphorylation can be used as markers for the mature fast and slow muscle phenotype. These include phosphorylase, phosphorylase kinase, and a cyclic adenosine 3′,5′‐monophosphate (cAMP)‐stimulated, calmodulin‐inhibited 28‐kDa band (markers for fast muscle), a calmodulin‐stimulated 50‐kDa band, and two cAMP‐stimulated bands at 44 and 46 kDa (markers for slow muscle), and the relative concentrations of the regulatory subunits of cAMP‐dependent protein kinase (RI and RII). After denervation the pattern of phosphorylation in fast muscle changed to resemble that of slow muscle: phosphorylation of the fast phenotype markers decreased; the slow phenotype markers, barely detectable in normal fast muscle, appeared as significant phosphoproteins; and the concentration of RII increased with no change in RI. This is consistent with denervation‐induced changes observed using other phenotypic markers and indicates the potential for using these phosphoprotein markers in studies of muscle development and pathophysiology. © 1998 John Wiley & Sons, Inc. Muscle Nerve 21:504–513, 1998. |
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Bibliography: | ArticleID:MUS9 The National Health and Medical Research Council of Australia ark:/67375/WNG-DRWQBVSB-9 University of Newcastle istex:192F7EFC4BB4E10A1A87B659D862022192CD09E3 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0148-639X 1097-4598 |
DOI: | 10.1002/(SICI)1097-4598(199804)21:4<504::AID-MUS9>3.0.CO;2-D |