Cyclic mechanical strain alters tissue-factor activity in rat osteosarcoma cells cultured on a titanium substrate
Tissue factor (TF), a transmembrane glycoprotein, plays a role in the initiation of blood coagulation at sites of vascular injury. Activated products of coagulation may then enhance inflammatory responses. The present investigation assesses the ability of rat osteosarcoma (UMR‐106) cells cultured on...
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Published in | Journal of biomedical materials research. Part A Vol. 70A; no. 3; pp. 490 - 496 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
01.09.2004
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Subjects | |
Online Access | Get full text |
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Summary: | Tissue factor (TF), a transmembrane glycoprotein, plays a role in the initiation of blood coagulation at sites of vascular injury. Activated products of coagulation may then enhance inflammatory responses. The present investigation assesses the ability of rat osteosarcoma (UMR‐106) cells cultured on titanium alloy (Ti6Al4V) to express differential surface TF activity in response to cyclic mechanical strain. Strains ranged from −2000 μ‐strain to +2000 μ‐strain, and durations from 5, 10, and 20 min per day over 5 days to 24 h continuous stimulation. ROS cells exhibited significant TF activity as demonstrated by the conversion of Factor X to Factor Xa. Strains of +2000 μ‐strain with 5–20‐min duration exhibited decreased TF activity with duration from 1.4E‐04 nM/cell to 8.7E‐05 nM/cell. Additionally, ROS cells stimulated with calcium ionophore (A23187) exhibited at least twice the activity of nonstimulated cells. Strains of +1340 μ‐strain with 5–20‐min duration exhibited an increasing trend with 4.15E‐05 nM/cell to 7.38E‐05 nM/cell. Strain direction had no significant effect on TF activity. Thus, both mechanical and chemical stimuli induce differential expression of TF activity by ROS cells cultured on Ti6Al4V, a phenomenon that may potentiate or regulate the inflammatory responses associated with the implantation of orthopedic biomaterials. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 70A: 490–496, 2004 |
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Bibliography: | ark:/67375/WNG-PDW2TLPC-4 istex:6E87B7B4A59DCA46BD1661AD1C9475AECA0EB43B ArticleID:JBM30108 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1549-3296 1552-4965 |
DOI: | 10.1002/jbm.a.30108 |