Platelet-activating factor induces a concentration-dependent spectrum of functional responses in bovine neutrophils

• Published in: Journal of leukocyte biology Vol. 64; no. 6; pp. 817 - 827
• Main Authors: Swain, Steve D., Bunger, Peggy L., Sipes, Karen M., Nelson, Laura K., Jutila, Kathryn L., Boylan, Shane M., Quinn, Mark T.
• Format: Journal Article
• Language: English
• Published: United States Society for Leukocyte Biology 01.12.1998
• Subjects: actin; Actins - metabolism; Animals; Blood Cells - cytology; Blood Cells - metabolism; Calcium - metabolism; Cattle; Cell Adhesion Molecules - metabolism; Cell Degranulation - drug effects; degranulation; Dose-Response Relationship, Drug; lactoferrin; membrane potential; Membrane Potentials - drug effects; Neutrophils - drug effects; Neutrophils - metabolism; Neutrophils - physiology; Platelet Activating Factor - metabolism; Platelet Activating Factor - physiology; priming; reactive oxygen species; Reactive Oxygen Species - metabolism; Respiratory Burst - drug effects
• ISSN: 0741-5400; 1938-3673
• DOI: 10.1002/jlb.64.6.817

We characterized the dose response of bovine neutrophils to platelet‐activating factor (PAF) with respect to the following functions: calcium flux and membrane potential changes, actin polymerization, degranulation, and the production and/or priming of the oxidative burst. PAF at very low concentrations (10 and 10 M) caused changes in intracellular calcium and membrane potential in bovine neutrophils, whereas moderate PAF concentrations (≥10–7 M) resulted in increased actin polymerization. Degranulation responses to PAF were more complex: low concentrations (10–9 M) caused secretory granule degranulation, moderate doses (≥ 10–7 M) caused specific granule degranulation, whereas azurophil degranulation only occurred at high (10–5 M) PAF concentrations. Treatment of bovine neutrophils with PAF at concentrations ≥10–7 M also caused up‐regulation of the adhesion molecules Mac‐1 and L‐selectin. PAF stimulation resulted in a very weak [compared to phorbol myristate acetate (PMA)] oxidative burst in bovine neutrophils, and only at high (10–6 M) concentrations. Unlike human neutrophils, bovine neutrophils were poorly primed by PAF treatment. Only high concentrations of PAF (10–5 M) caused an increased rate of PMA‐stimulated superoxide production, although lower doses of PAF did reduce the lag time preceding the PMA‐induced oxidative burst. The overall pattern that can be inferred is that lower concentrations of PAF promote neutrophil sensitivity and interaction by selective degranulation, up‐regulation of adhesion molecules, and increased actin polymerization. In contrast, higher PAF concentrations can promote, albeit weakly, more direct bactericidal responses, such as the release of reactive oxygen species and granule enzymes. The ability of PAF to modulate a graded response in bovine neutrophils would allow the cell to respond proportionally to the severity of a stimulus. J. Leukoc. Biol. 64: 817–827; 1998.