Immobilization of lipase on poly(N-vinyl-2-pyrrolidone-co-styrene) hydrogel

• Published in: Journal of applied polymer science Vol. 82; no. 6; pp. 1404 - 1409
• Main Authors: Basri, M., Harun, A., Ahmad, M. B., Razak, C. N. A., Salleh, A. B.
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 07.11.2001; Wiley
• Subjects: activation; Applied sciences; Biological and medical sciences; Biotechnology; Crosslinking; Enzyme immobilization; Esterification; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Hydrogels; Immobilization of enzymes and other molecules; Immobilization techniques; Leaching; lipase; Methods. Procedures. Technologies; Organic polymers; Physicochemistry of polymers; Polymerization; Properties and characterization; Special properties (catalyst, reagent or carrier); stabilization; Thermodynamic stability; Solvent effect; Support; Enzyme; Triacylglycerol lipase; Esterases; Experimental study; Esterification; Thermal stability; Carboxylic ester hydrolases; Fungi; Colloidal gel; Enzymatic activity; Storage stability; Preparation; Pyrrolidone(vinyl) copolymer; Hydrolases; Fungi Imperfecti; Hydrogel; Immobilized enzyme; Candida rugosa; Thallophyta; Styrene copolymer
• ISSN: 0021-8995; 1097-4628
• DOI: 10.1002/app.1977

Lipase from Candida rugosa was immobilized by entrapment while polymerizing a poly(N‐vinyl‐2‐pyrrolidone‐co‐styrene) [poly(VP‐co‐ST)] hydrogel using ethylene dimethacrylate (EDMA) as the crosslinking agent. The immobilized enzymes were used in the esterification reaction of oleic acid and butanol in hexane. The activities of the immobilized enzymes and the leaching ability of the enzyme from the support with respect to the different compositions of the hydrogels were investigated. The thermal, solvent, and storage stability of the immobilized lipases were also determined. The activities were relatively higher when the percent compositions of VP(%):ST(%) were 50:50 and 30:70. The lipase immobilized on VP(%):ST(%) 50:50 showed the highest thermal stability, while lipase immobilized on VP(%):ST(%) 30:70 exhibited the highest solvent stability. © 2001 John Wiley & Sons, Inc. J Appl Polym Sci 82: 1404–1409, 2001