In vivo photothermal flow cytometry: Imaging and detection of individual cells in blood and lymph flow

• Published in: Journal of cellular biochemistry Vol. 97; no. 5; pp. 916 - 932
• Main Authors: Zharov, Vladimir P., Galanzha, Ekaterina I., Tuchin, Valery V.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.04.2006
• Subjects: Animals; blood and lymph vessels; Cell Separation - methods; cells; flow cytometry; Flow Cytometry - methods; Gold - chemistry; gold labels; Humans; Image Interpretation, Computer-Assisted - methods; Lymph - physiology; Mesentery - blood supply; Mesentery - ultrastructure; Mice; molecular imaging; Photometry - methods; photothermal technique; rat mesentery; Rats; small animal; Spectrum Analysis - methods; Thermography - methods
• ISSN: 0730-2312; 1097-4644
• DOI: 10.1002/jcb.20766

Flow cytometry is a well‐established, powerful technique for studying cells in artificial flow in vitro. This review covers a new potential application of this technique for studying normal and abnormal cells in their native condition in blood or lymph flow in vivo. Specifically, the capabilities of the label‐free photothermal (PT) technique for detecting and imaging cells in the microvessel network of rat mesentery are analyzed from the point of view of overcoming the problems of flow cytometry in vivo. These problems include, among others, the influences of light scattering and absorption in vessel walls and surrounding tissues, instability of cell velocity, and cells numbers and positions in a vessel's cross‐section. The potential applications of this new approach in cell biochemistry and medicine are discussed, including molecular imaging; studying the metabolism and pathogenesis of many diseases at a cellular level; and monitoring and quantifying metastatic and apoptotic cells, and/or their responses to therapeutic interventions (e.g., drug or radiation), in natural biological environments. J. Cell. Biochem. 97: 916–932, 2006. © 2006 Wiley‐Liss, Inc.