Secretion of atherogenic risk factor apolipoprotein B-100 is increased by a potential mechanism of JNK/PKC-mediated insulin resistance in liver cells

• Published in: Journal of cellular biochemistry Vol. 103; no. 3; pp. 908 - 919
• Main Authors: Jin, Un-Ho, Kang, Yun-Jeong, Chang, Young-Chae, Kim, Cheorl-Ho
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 15.02.2008
• Subjects: Adaptor Proteins, Signal Transducing - metabolism; apolipoprotein B-100; Apolipoprotein B-100 - drug effects; Apolipoprotein B-100 - secretion; Carrier Proteins - drug effects; Carrier Proteins - metabolism; Cells, Cultured; dyslipidemia; Enzyme Inhibitors - chemistry; Enzyme Inhibitors - pharmacology; Hepatocytes - drug effects; Hepatocytes - metabolism; Humans; Insulin Receptor Substrate Proteins; insulin resistance; Insulin Resistance - physiology; JNK Mitogen-Activated Protein Kinases - metabolism; Lipoproteins, VLDL - drug effects; Lipoproteins, VLDL - metabolism; Metabolic Syndrome - metabolism; phorbol 12-myristate-13-acetate; Phosphorylation - drug effects; Protein Kinase C - metabolism; Protein-Serine-Threonine Kinases - metabolism; Risk Factors; Serine - chemistry; Tetradecanoylphorbol Acetate - metabolism; Tetradecanoylphorbol Acetate - pharmacology; Vanadates - chemistry; Vanadates - pharmacology
• ISSN: 0730-2312; 1097-4644
• DOI: 10.1002/jcb.21462

Apolipoprotein B‐100 (ApoB) is the main protein of the atherogenic lipoproteins and plasma ApoB levels reflect the total numbers of atherogenic lipoproteins. Induction of insulin resistance was accompanied by a considerable rise in the production of hepatic very low density lipoprotein (VLDL) containing ApoB and triglyceride. Increased plasma levels of ApoB and triglyceride in VLDL are common characteristics of the dyslipidemia associated with insulin resistance and type 2 diabetes mellitus. Thus, we investigate whether phorbol 12‐myristate‐13‐acetate (PMA)‐induced insulin resistance affects the increase of ApoB secretion. PMA increased ApoB secretion and transcriptional level of microsomal triglyceride transfer protein (MTP). PMA treatment also resulted in increase of insulin receptor substrate 1 (IRS1) serine312 (Ser312) and serine1101 (Ser1101) phosphorylation and induction of IRS1 degradation. Additionally, PMA induced activation of c‐jun N‐terminal kinase (JNK) and protein kinase C (PKC) isoforms (α, βI, δ, ζ, θ), and reduced AKT8 virus oncogene cellular homolog (AKT) activation in a time dependent manner. PMA‐induced ApoB secretion, MTP promoter activities, and IRS1 degradation was significantly decreased by treatment of JNK and PKCs inhibitors. Orthovanadate, a potent tyrosine phosphatase inhibitor, increased tyrosine phosphorylation of IRS1 and decreased ApoB secretion of Chang liver cells although PMA was co‐treated. From the results, it was concluded that PMA‐induced insulin resistance, through induction of serine phosphorylation of IRS1 mediated by activated JNK and PKCs, increases ApoB secretion in Chang liver cells. J. Cell. Biochem. 103: 908–919, 2008. © 2007 Wiley‐Liss, Inc.