Characterization of skeletal muscle effects associated with daptomycin in rats

• Published in: Muscle & nerve Vol. 42; no. 3; pp. 385 - 393
• Main Authors: Kostrominova, Tatiana Y., Hassett, Cheryl A., Rader, Erik P., Davis, Carol, Larkin, Lisa M., Coleman, Scott, Oleson, Frederick B., Faulkner, John A.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.09.2010; Wiley
• Subjects: Animals; Anti-Bacterial Agents - administration & dosage; Anti-Bacterial Agents - toxicity; Biological and medical sciences; Body Weight - drug effects; caveolin-3; Cell Membrane - drug effects; Cell Membrane - physiology; Cranial nerves. Spinal roots. Peripheral nerves. Autonomic nervous system. Gustation. Olfaction; daptomycin; Daptomycin - administration & dosage; Daptomycin - toxicity; dysferlin; Evans Blue; Fundamental and applied biological sciences. Psychology; Immunohistochemistry; Injections, Intravenous; LAMP-1; Male; Medical sciences; Microscopy, Fluorescence; Muscle Contraction - drug effects; Muscle Contraction - physiology; Muscle Fibers, Skeletal - drug effects; Muscle, Skeletal - drug effects; Muscle, Skeletal - pathology; Muscle, Skeletal - physiology; Nervous system (semeiology, syndromes); Neurology; Organ Size - drug effects; Rats; Rats, Sprague-Dawley; sarcolemma; Sarcolemma - drug effects; Striated muscle. Tendons; Vertebrates: osteoarticular system, musculoskeletal system; Sarcolemma; Rat; Rodentia; Granulocyte; Muscular fiber; Striated muscle; Vertebrata; Mammalia; LAMP-1; dysferlin; Plasma membrane; caveolin-3; daptomycin; Repair; Macrophage
• ISSN: 0148-639X; 1097-4598
• DOI: 10.1002/mus.21691

Daptomycin is a lipopeptide antibiotic with strong bactericidal effects against Gram‐positive bacteria and minor side effects on skeletal muscles. The type and magnitude of the early effect of daptomycin on skeletal muscles of rats was quantified by histopathology, examination of contractile properties, Evans Blue Dye uptake, and effect on the patch repair process. A single dose of daptomycin of up to 200 mg/kg had no effect on muscle fibers. A dose of 150 mg/kg of daptomycin, twice per day for 3 days, produced a small number of myofibers (≤0.22%) with loss of plasma membrane integrity and/or infiltration by neutrophils and/or macrophages. Multiple doses of daptomycin are required for a quantifiable effect on skeletal muscles of rats. Some fibers were Evans Blue Dye–positive but were not yet infiltrated by neutrophils. This suggests that the sarcolemma may be the primary target for the observed effects. Muscle Nerve, 2010