Impact of secondary reactive species on the apparent decoupling of poly(ethylene glycol) diacrylate hydrogel average mesh size and modulus

• Published in: Polymer (Guilford) Vol. 77; pp. 227 - 238
• Main Authors: Munoz-Pinto, Dany J., Samavedi, Satyavrata, Grigoryan, Bagrat, Hahn, Mariah S.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 23.10.2015
• Subjects: Biotechnology; Controlled release; Decoupling; Formulations; Glycols; Hydrogels; Mesh size; Modulus; PEGDA; Poly(ethylene glycol) hydrogels; Three dimensional; Vices; PEGDA; Decoupling; Poly(ethylene glycol) hydrogels; Mesh size; Modulus
• ISSN: 0032-3861; 1873-2291
• DOI: 10.1016/j.polymer.2015.09.032

Poly(ethylene glycol) diacrylate (PEGDA) hydrogels are widely used in biotechnology due to their in situ crosslinking capacity and tunable physical properties. However, as with all single component hydrogels, the modulus of PEGDA networks cannot be tailored independently of mesh size. This interdependence places significant limitations on their use for defined, 3D cell-microenvironment studies and for certain controlled release applications. The incorporation of secondary reactive species (SRS) into PEGDA hydrogels has previously been shown to allow the identification of up to 6 PEGDA hydrogel formulations for which distinct moduli can be obtained at consistent average mesh size (or vice versa). However, the modulus and mesh size ranges which can be probed by these formulations are quite restricted. This work presents an in-depth study of SRS incorporation into PEGDA hydrogels, with the goal of expanding the space for which “decoupled” examination of modulus and mesh size effects is achievable. Towards this end, over 100 PEGDA hydrogels containing either N-vinyl pyrrolidone or star PEG-tetraacrylate as SRS were characterized. To our knowledge, this is the first study to demonstrate that SRS incorporation allows for the identification of a number of modulus ranges that can be probed at consistent average mesh size (or vice versa). Inclusion of secondary reactive species (SRS) within PEGDA hydrogels results in shifts in the network modulus-mesh size curves, with the degree of shift increasing with decreasing PEGDA molecular weight. These shifts result in apparent “decoupling” between modulus and mesh size, allowing a number of modulus ranges to be examined at consistent average mesh size (or vice versa). [Display omitted] •We examined NVP and PEGTA as secondary reactive species (SRS) within PEGDA networks.•NVP and PEGTA both caused in substantive shifts in the modulus-mesh size curves of PEGDA hydrogels.•The effects of both NVP and PEGTA on apparent decoupling were dose dependent.