Boundary-element calculations for dielectric behavior of doublet-shaped cells

• Published in: Biochimica et biophysica acta Vol. 1721; no. 1; pp. 130 - 138
• Main Authors: Sekine, Katsuhisa, Watanabe, Yoko, Hara, Saori, Asami, Koji
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 18.01.2005
• Subjects: Cell Polarity; Clausius–Mossotti factor; Complex permittivity; Dielectric relaxation; Interfacial polarization; Saccharomyces cerevisiae; Saccharomyces cerevisiae - cytology; Simulation; Suspensions; Interfacial polarization; Clausius–Mossotti factor; Simulation; Complex permittivity; Saccharomyces cerevisiae; Dielectric relaxation
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/j.bbagen.2004.10.010

In order to simulate dielectric relaxation spectra (DRS) of budding yeast cells ( Saccharomyces cerevisiae) in suspension, the complex polarization factor (Clausius–Mossotti factor) β for a single cell and the complex permittivity of a cell suspension ε sus* were calculated with a doublet-shaped model (model RD), in which two spheres were connected with a part of a ring torus, using the boundary element method. The β values were represented by a diagonal tensor consisting of components β z parallel to the rotation axis ( z axis) and β h in a plane ( h plane) perpendicular to the axis. The ε sus* values were calculated from the complex permittivity of the suspending medium ε a* and the components of β. The calculation was compared with that of a conventional prolate spheroid model (model CP). It was found that model CP could be used as a first approximation to model RD. However, differences existed in β z between models RD and CP; β z showed three relaxation terms in the case of model RD in contrast with two terms in model CP. Narrowing the junction between the two spheres in model RD markedly decreased the characteristic frequency of one of the relaxation terms in β z. This suggests that the structure of the junction can be estimated from DRS. Effects of the shape change from model RD to a two-sphere model (model RD without the junction) were also examined. The behavior of β z in the two-sphere model, the relaxation intensity of which was much lower than model RD, was quite similar to that in a single-sphere model. These simulations were consistent with the experimental observations of the dielectric behavior of the yeast cells during cell cycle progression.