Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses

• Published in: Archives of virology Vol. 134; no. 3/4; pp. 421 - 426
• Main Authors: Zhang, X, Herbst, W, Kousoulas, K.G, Storz, J
• Format: Journal Article
• Language: English
• Published: Austria 01.01.1994
• Subjects: Agglutination Tests; Amino Acid Sequence; amino acid sequences; Animals; Antibodies, Monoclonal - immunology; antigen-antibody reactions; antigenic variation; Base Sequence; Bovidae; Cattle; Cattle Diseases - microbiology; Cell Line; Chickens; Coronavirus; Coronavirus Infections - microbiology; Coronavirus Infections - veterinary; Coronavirus, Bovine - genetics; Coronavirus, Bovine - immunology; Cytopathogenic Effect, Viral; DNA, Viral; Enteritis - microbiology; Enteritis - veterinary; genes; Genes, Viral; Glycoproteins - genetics; Lung Diseases - microbiology; Lung Diseases - veterinary; Membrane Glycoproteins; Mice; Molecular Sequence Data; monoclonal antibodies; nucleotide sequences; sequence analysis; Spike Glycoprotein, Coronavirus; Viral Envelope Proteins - genetics
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/BF01310579

The nucleotide sequence of the S gene of the bovine respiratory coronavirus (BRCV) strain G95, which was isolated from nasal swabs of a calf suffering from respiratory disorders, was determined and compared with the S gene of the enteropathogenic bovine coronavirus (BECV) strain LY138. Sequence analysis revealed 98.7% nucleotide and 98.3% deduced amino acid identities between the S genes of BRCV-G95 and BECV-LY138 without any deletions or insertions. Nucleotide substitutions were distributed randomly throughout the gene. Five monoclonal antibodies specific for the S protein distinguished BRCV-G95 from BECV-L9, but failed to differentiate it from BECV-LY 138 in Western blots under denatured and native conditions. BRCV-G95 induced cytopathic changes in cell cultures that were similar to BECV-LY138 but different from BECV-L9. These results suggest that strain BRCV-G95 is more closely related to the virulent strain BECV-LY138 than to the avirulent, cell-culture-adapted strain BECV-L9.