Purified Capsular Polysaccharide of Neisseria meningitidis Serogroup A as Immune Potentiator for Antibody Production

• Published in: Current microbiology Vol. 60; no. 2; pp. 79 - 84
• Main Authors: Menéndez, Tamara, Carmenate, Tania, Cruz-Leal, Yoelys, Coizeau, Edelgis, Caballero, Evelyn, Bello, Daniel, Guirola, María, Álvarez, Anabel, Guillén, Gerardo
• Format: Journal Article
• Language: English
• Published: New York New York : Springer-Verlag 01.02.2010; Springer-Verlag; Springer Nature B.V
• Subjects: Adjuvants, Immunologic - administration & dosage; Adjuvants, Immunologic - isolation & purification; Animal models; Animals; Antibodies, Bacterial - blood; Antibodies, Bacterial - immunology; Antigens; Bacteria; Bacterial Capsules - administration & dosage; Bacterial Capsules - immunology; Bacterial Capsules - isolation & purification; Biomedical and Life Sciences; Biotechnology; Blood Bactericidal Activity; Capsular polysaccharides; Carbohydrates; Female; Humans; Immunization; Immunoglobulin G; Immunoglobulin G - blood; Immunoglobulin G - immunology; Life Sciences; Meningitis, Meningococcal - immunology; Meningitis, Meningococcal - microbiology; Meningitis, Meningococcal - prevention & control; meningococcal disease; Meningococcal Vaccines - administration & dosage; Meningococcal Vaccines - immunology; Mice; Mice, Inbred BALB C; Microbiology; Neisseria meningitidis; Neisseria meningitidis, Serogroup A - chemistry; Neisseria meningitidis, Serogroup A - immunology; Polysaccharides, Bacterial - administration & dosage; Polysaccharides, Bacterial - immunology; Random Allocation; Vaccines; Serum Bactericidal Activity; Outer Membrane Vesicle; IgG2a Antibody; Capsular Polysaccharide; Neisseria Meningitidis
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-009-9505-9

The development of new immune potentiators for human vaccines is an important and expanding field of research. In the present study, the ability of the capsular polysaccharide from Neisseria meningitidis serogroup A (CPS-A), a mannose-containing carbohydrate, to enhance the antibody production against a co-administered model vaccine antigen, is examined. A protein-meningococcal serogroup C capsular polysaccharide (CPS-C) conjugate was selected as the model antigen for this study. After subcutaneous immunization of Balb/C mice, the conjugate mixed with CPS-A induced higher anti-CPS-C IgG and IgG₂a antibody levels and higher anti-meningococcal serogroup C bactericidal titers than the conjugate alone or mixed with CPS-C. The immuno-stimulatory properties exhibited by CPS-A and the fact that vaccines based on purified CPS-A has been safely used during decades to fight the serogroup A meningococcal disease, support the proposal to use CPS-A as immune potentiator for human vaccination studies.