Co-culturing of Lactobacillus paracasei subsp. paracasei with a Lactobacillus delbrueckii subsp. delbrueckii Mutant to Make High Cell Density for Increased Lactate Productivity from Cassava Bagasse Hydrolysate

• Published in: Current microbiology Vol. 62; no. 3; pp. 790 - 794
• Main Authors: John, Rojan Pappy, Madhavan Nampoothiri, K
• Format: Journal Article
• Language: English
• Published: New York New York : Springer-Verlag 01.03.2011; Springer-Verlag; Springer Nature B.V
• Subjects: Acid production; Bacteria; Bagasse; Biochemistry; Biofilms; Biomedical and Life Sciences; Biotechnology; Biotechnology - methods; Cells, Immobilized - metabolism; Cellulose - metabolism; Chemical compounds; Fermentation; Lactic Acid - metabolism; Lactobacillus; Lactobacillus - growth & development; Lactobacillus - metabolism; Lactobacillus delbrueckii; Lactobacillus paracasei; Life Sciences; Manihot - metabolism; Manihot esculenta; Microbiology; Mutation; Alginate; Fermentation; Lactic Acid; Lactobacillus; Cassava
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-010-9793-0

To increase the productivity of lactic acid, a co-culture of lactobacilli was made by mixing 1:1 ratio of Lactobacillus paracasei subsp. paracasei and a fast growing L. delbrueckii subsp. delbrueckii mutant. The culture was embedded on to polyurethane foam (PUF) cubes as a biofilm and used for fermentation. In order to prevent the cell leakage, the PUF cubes were further entrapped in calcium cross-linked alginate. The maximum lactic acid production using a high cell density free culture was >38 g l⁻¹ from ~40 g l⁻¹ of reducing sugar within 12 h of fermentation. Using PUF biofilms, the same yield of lactic acid attained after 24 h. When the cubes were further coated with alginate it took 36 h for the maximum yield. Even though, the productivity is slightly lesser with the alginate coating, cell leakage was decreased and cubes were reused without much decrease in production in repeated batches. Using a conventional control inoculum (3%, w/v), it took 120 h to yield same amount of lactic acid.