Amycolatopsis marina sp. nov., an actinomycete isolated from an ocean sediment

• Published in: International journal of systematic and evolutionary microbiology Vol. 59; no. 3; pp. 477 - 481
• Main Authors: Bian, Jiang, Li, Yan, Wang, Jian, Song, Fu-Hang, Liu, Mei, Dai, Huan-Qin, Ren, Biao, Gao, Hong, Hu, Xinling, Liu, Zhi-Heng, Li, Wen-Jun, Zhang, Li-Xin
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.03.2009; Society for General Microbiology
• Subjects: Actinomycetales - chemistry; Actinomycetales - classification; Actinomycetales - genetics; Actinomycetales - isolation & purification; Amycolatopsis; Bacteriology; Biological and medical sciences; China; DNA, Bacterial - analysis; DNA, Ribosomal Spacer - analysis; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Geologic Sediments - microbiology; ISEW, South China Sea; Microbiology; Miscellaneous; Molecular Sequence Data; Nucleic Acid Hybridization; Phylogeny; RNA, Ribosomal, 16S - genetics; RNA, Ribosomal, 23S - genetics; Seawater - microbiology; Sequence Analysis, DNA; Species Specificity; Systematics; China; Taxonomy; Amycolatopsis; Bacteria; Actinomycetes; New species; Pseudonocardiaceae; Sediments
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.000026-0

1 Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China 2 South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China 3 Graduate University of the Chinese Academy of Sciences, Beijing 100049, PR China 4 Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China 5 Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510663, PR China 6 SynerZ Pharmaceuticals Inc., Lexington, MA 02421, USA Correspondence Li-Xin Zhang zhanglixin{at}im.ac.cn A Gram-positive, aerobic, non-motile actinobacterium, designated strain Ms392A T , was isolated from an ocean-sediment sample collected from the South China Sea. The isolate contained chemical markers that supported chemotaxonomic assignment to the genus Amycolatopsis . On the basis of an analysis of 16S rRNA gene sequence similarities, strain Ms392A T represents a novel subclade within the genus Amycolatopsis , with Amycolatopsis palatopharyngis 1BDZ T as its closest phylogenetic neighbour (99.4 % similarity). However, DNA–DNA hybridization demonstrated that strain Ms392A T was distinct from A. palatopharyngis AS 4.1729 T (48.6 % relatedness). The polyphasic analysis demonstrated that the ocean isolate can be clearly distinguished from recognized species of the genus Amycolatopsis . Therefore, strain Ms392A T represents a novel species of the genus Amycolatopsis , for which the name Amycolatopsis marina sp. nov. is proposed. The type strain is Ms392A T (=CGMCC 4.3568 T =NBRC 104263 T ). These authors contributed equally to this work. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain Ms392A T is EU329845 .