Long non-coding RNA RAD51-AS1 promotes the tumorigenesis of ovarian cancer by elevating EIF5A2 expression

• Published in: Journal of cancer research and clinical oncology Vol. 150; no. 4; p. 179
• Main Authors: Zhao, Lu, Huang, Jia, Liu, Wenting, Su, Xiaoyan, Zhao, Bei, Wang, Xianggang, He, Xiaoju
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 07.04.2024; Springer Nature B.V
• Subjects: Antisense RNA; Apoptosis; Bioinformatics; Cancer Research; Carcinogenesis - genetics; Cell cycle; Cell Line, Tumor; Cell Movement - genetics; Cell proliferation; Cell Proliferation - genetics; Cell Transformation, Neoplastic - genetics; DNA repair; Female; Gene expression; Gene Expression Regulation, Neoplastic; Genomes; Hematology; Humans; Initiation factor eIF-5A; Internal Medicine; Medicine; Medicine & Public Health; Metastases; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; Molecular modelling; Oncology; Ovarian cancer; Ovarian Neoplasms - genetics; Rad51 Recombinase - genetics; RNA, Long Noncoding - genetics; Therapeutic targets; Tumorigenesis; Western blotting; RAD51-AS1; miR-140-3p; EIF5A2; Ovarian cancer
• ISSN: 1432-1335; 0171-5216; 1432-1335
• DOI: 10.1007/s00432-024-05671-z

Purpose The present study aims to determine the molecular mechanism mediated by RAD51 antisense RNA 1 (RAD51-AS1) in ovarian cancer (OvCA). Methods The data associated with RAD51-AS1 in OvCA were obtained from the Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) database. Relative expression of RAD51-AS1 was detected. Determination of cell proliferation, metastasis, and invasion was performed by cell counting, colony formation, would-healing, and transwell invasion assays. Protein levels were detected by western blotting. The molecular mechanism mediated by RAD51-AS1 was predicted by bioinformatics analysis and verified by dual-luciferase reporter assays. Subcutaneous tumorigenesis models were used to confirm the function of RAD51-AS1 in vivo. Results Data from TCGA and GEO showed that RAD51-AS1 was associated with poor prognosis in OvCA patients and DNA repair, cell cycle, focal adhesion, and apoptosis in SKOV3.ip cells. High levels of RAD51-AS1 were detected in OvCA cells. Overexpressing RAD51-AS1 enhanced the proliferative, invading, and migratory capabilities of OvCA cells in vitro while silencing RAD51-AS1 exhibited the opposite effects. Mechanically, RAD51-AS1 elevated eukaryotic initiation factor 5A2 (EIF5A2) expression as a sponge for microRNA (miR)-140-3p. Finally, the role of RAD51-AS1 was verified by subcutaneous tumorigenesis models. Conclusion RAD51-AS1 promoted OvCA progression by the regulation of the miR-140-3p/EIF5A2 axis, which illustrated the potential therapeutic target for OvCA.