Synergistic Interaction Effect of Artemisia cina n-hexane Extract and Tagetes lucida Ethyl Acetate Extract on Haemonchus Contortus

• Published in: Acta parasitologica Vol. 69; no. 2; pp. 1132 - 1140
• Main Authors: Santiago-Figueroa, Itzel, González-Cortazar, Manases, Estrada-Flores, Julieta Gertrudis, Cuéllar-Ordaz, Jorge Alfredo, López-Arellano, María Eugenia, González-Reyes, Francisco Javier, Olmedo-Juárez, Agustín, Higuera-Piedrahita, Rosa Isabel
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.06.2024; Springer Nature B.V
• Subjects: Acetates - pharmacology; Acetic acid; Animal Systematics/Taxonomy/Biogeography; Animals; Anthelmintics - pharmacology; Antiparasitic agents; Artemisia - chemistry; Artemisia cina; Biomedical and Life Sciences; Biomedicine; Drug Synergism; Ecology; Eggs; Ethyl acetate; Haemonchiasis - drug therapy; Haemonchiasis - parasitology; Haemonchiasis - veterinary; Haemonchus - drug effects; Hatching; Hexanes; Larva - drug effects; Larvae; Medical Microbiology; Microbiology; n-Hexane; Original Paper; Ovum - drug effects; Parasitology; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant species; Regression analysis; Sheep; Sheep Diseases - drug therapy; Sheep Diseases - parasitology; Synergistic effect; Tagetes - chemistry; Tagetes lucida; Variance analysis; Sustainable Anthelmintic; Small Ruminants; Bioactive Plants; Pharmacology Interaction; Gastroenteric Nematodes
• ISSN: 1230-2821; 1896-1851; 1896-1851
• DOI: 10.1007/s11686-024-00839-6

Purpose We analysed the possible synergistic activity among active extracts from Artemisia cina and Tagetes lucida combinations on Haemonchus contortus , a nematode parasitising sheep. Methods The work was carried out in vitro on eggs and infective larvae (L3) of H. contortus . The results were analysed with SAS 9.1, applying the ANOVA and Tukey test, and the lethal concentration (LC) values LC50 and LC90 were determined with regression analysis, employing Proc Probit of SAS 9.1. Additionally, the lethal concentration (LC) was calculated with LC 50 and LC 90 to determine the synergistic effect. Results The results demonstrated a high efficacy of the two plants studied on both nematode eggs and L3 larvae as well as of their combinations. The highest egg hatching inhibition was obtained with a 50/50 combination, and the best larvae mortality was obtained with 25% A. cina and 75% T. lucida at 10 mg/mL. Additionally, this combination showed a synergistic effect. Conclusion The two plant species studied here can be applied as natural anthelmintic alternatives due to their high bioactive effect and synergistic response.