Identification of a major QTL for flag leaf glaucousness using a high-density SNP marker genetic map in hexaploid wheat

• Published in: Journal of Integrative Agriculture Vol. 16; no. 2; pp. 445 - 453
• Main Authors: LI, Chun-lian, LI, Ting-ting, LIU, Tian-xiang, SUN, Zhong-pei, BAI, Gui-hua, JIN, Feng, WANG, Yong, WANG, Zhong-hua
• Format: Journal Article
• Language: English
• Published: KeAi Communications Co., Ltd 01.02.2017
• Subjects: abiotic stress; alleles; chromosome mapping; chromosomes; cultivars; epicuticular wax; flag leaf glaucousness; genetic distance; genetic markers; hexaploidy; inbred lines; leaves; microsatellite repeats; phenotypic variation; QTL; QTL×Environment interactions; quantitative trait loci; quantitative trait locus (QTL); single nucleotide polymorphism; SNP; Triticum aestivum; wheat; 单核苷酸多态性; 小麦; 旗叶; 标记; 遗传图谱; 高密度
• ISSN: 2095-3119; 2352-3425
• DOI: 10.1016/S2095-3119(16)61339-4

Cuticular wax plays an important role in protecting land plant against biotic and abiotic stresses. Cuticular wax production on plant surface is often visualized by a characteristic glaucous appearance. This study identified quantitative trait loci （QTLs） for wheat （Triticum aestivum L.） flag leaf glaucousness （FLG） using a high-density genetic linkage map developed from a recombinant inbred line （RIL） population derived from the cross HeynexLakin by single-seed descent. The map consisted of 2 068 single nucleotide polymorphism （SNP） markers and 157 simple sequence repeat （SSR） markers on all 21 wheat chromosomes and covered a genetic distance of 2 381.19 cM, with an average marker interval of 1.07 cM. Two additive QTLs for FLG were identified on chromosomes 3AL and 2DS with the increasing FLG allele contributed from Lakin. The major QTL on 3AL, QFIg.hwwgr-3AL, explained 17.5-37.8% of the phenotypic variation in different environments. QFIg.hwwgr-3AL was located in a 4.4-cM interval on chromosome 3AL that was flanked by two markers IWA1831 and IWA8374. Another QTL for FLG on 2DS, designated as QFIg.hwwgr-2DS which was identified only in Yangling in 2014 （YL14）, was flanked by IWA1939 and Xgwm261 and accounted for 11.3% of the phenotypic variation for FLG. QFIg.hww- gr-3AL and QFIg.hwwgr-2DS showed Additive×Environment （AE） interactions, explaining 3.5 and 4.4% of the phenotypic variance, respectively. Our results indicated that different genes/QTLs may contribute different scores of FLG in a cultivar and that the environment may play a role in FLG.