Role of P-Selectin in Platelet Sequestration in Pulmonary Capillaries during Endotoxemia

• Published in: Journal of vascular research Vol. 43; no. 5; pp. 473 - 481
• Main Authors: Kiefmann, Rainer, Heckel, Kai, Schenkat, Sonja, Dörger, Martina, Goetz, Alwin Eduard
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland Karger 01.01.2006; S. Karger AG
• Subjects: Animals; Biological and medical sciences; Blood Platelets - physiology; Capillaries - physiopathology; Disease Models, Animal; Endotoxemia - blood; Endotoxemia - physiopathology; Erythrocytes - physiology; Fundamental and applied biological sciences. Psychology; Kinetics; P-Selectin - blood; P-Selectin - physiology; Pulmonary Circulation - physiology; Rabbits; Research Paper; Vertebrates: cardiovascular system; Acute lung injury; Alveolar capillary networks; Platelets; Endotoxemia; P-selectin; Lung disease; Respiratory disease; Acute; Cardiovascular disease; Pulmonary capillary; Vascular disease; P Selectin; Vertebrata; Platelet; Mammalia; Malformation; Pulmonary sequestration; Network; Circulatory system
• ISSN: 1018-1172; 1423-0135
• DOI: 10.1159/000095247

Background: There is growing evidence that platelets accumulate in the lung and contribute to the pathogenesis of acute lung injury during endotoxemia. The aims of the present study were to localize platelet sequestration in the pulmonary microcirculation and to investigate the role of P-selectin as a molecular mechanism of platelet endothelial cell interaction. Methods: We used in vivo fluorescence microscopy to quantify the kinetics of fluorescently labeled erythrocytes and platelets in alveolar capillary networks in rabbit lungs. Results: Six hours after onset of endotoxin infusion we observed a massive rolling along and firm adherence of platelets to lung capillary endothelial cells whereas under control conditions no platelet sequestration was detected. P-selectin was expressed on the surface of separated platelets which were incubated with endotoxin and in lung tissue. Pretreatment of platelets with fucoidin, a P-selectin antagonist, significantly attenuated the endotoxin-induced platelet rolling and adherence. In contrast, intravenous infusion of fucoidin in endotoxin-treated rabbits did not inhibit platelet sequestration in pulmonary capillaries. Conclusion: We conclude that platelets accumulate in alveolar capillaries following endotoxemia. P-selectin expressed on the surface of platelets seems to play an important role in mediating this platelet-endothelial cell interaction.