Reconstruction of microbial transcriptional regulatory networks

• Published in: Current opinion in biotechnology Vol. 15; no. 1; pp. 70 - 77
• Main Authors: Herrgård, Markus J, Covert, Markus W, Palsson, Bernhard Ø
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2004
• Subjects: Bacteria - genetics; Bacteria - metabolism; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Databases, Protein; Evolution, Molecular; Gene Expression Profiling - methods; Gene Expression Regulation, Bacterial - physiology; Genome, Bacterial; Models, Biological; Signal Transduction - physiology; Transcription, Genetic - physiology; GWLA; TF
• ISSN: 0958-1669; 1879-0429
• DOI: 10.1016/j.copbio.2003.11.002

Although metabolic networks can be readily reconstructed through comparative genomics, the reconstruction of regulatory networks has been hindered by the relatively low level of evolutionary conservation of their molecular components. Recent developments in experimental techniques have allowed the generation of vast amounts of data related to regulatory networks. This data together with literature-derived knowledge has opened the way for genome-scale reconstruction of transcriptional regulatory networks. Large-scale regulatory network reconstructions can be converted to in silico models that allow systematic analysis of network behavior in response to changes in environmental conditions. These models can further be combined with genome-scale metabolic models to build integrated models of cellular function including both metabolism and its regulation.