Differential effects of low and high temperature stress on pollen germination and tube length of mango (Mangifera indica L.) genotypes

• Published in: Scientific reports Vol. 13; no. 1; p. 611
• Main Authors: Liu, Xinyu, Xiao, Yilin, Zi, Jing, Yan, Jing, Li, Chunhong, Du, Chengxun, Wan, Jiaxin, Wu, Hongxia, Zheng, Bin, Wang, Songbiao, Liang, Qingzhi
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 12.01.2023; Nature Publishing Group UK; Nature Portfolio
• Subjects: Catalase; Flowering; Genotype; Genotypes; Germination; Heat resistance; Heat-Shock Response; High temperature; Hot Temperature; Low temperature; Mangifera - anatomy & histology; Mangifera - genetics; Mangifera - growth & development; New varieties; Plant Breeding; Pollen; Pollen Tube - anatomy & histology; Pollen Tube - growth & development; Pollen tubes; Superoxide dismutase; Temperature
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-27917-5

Mango flowering is highly sensitive to temperature changes. In this research, the maximum values of pollen germination rate (PGR), pollen tube length (PTL) and their cardinal temperatures (Tmin, Topt and Tmax) were estimated by using quadratic equation and modified bilinear model under the conditions of 14-36 °C. The pollen germination rate in four mango varieties ranged from 29.1% ('Apple mango') to 35.5% ('Renong No. 1'); the length of pollen tube ranged from 51.2 μm ('Deshehari') to 56.6 μm ('Jinhuang'). The cardinal temperatures ranges (Tmin, Topt and Tmax) of pollen germination were 20.3-22.8 °C, 26.7-30.6 °C and 30.4-34.3 °C, respectively; similarly, cardinal temperatures (Tmin, Topt and Tmax) of pollen tube growth were 20.3-21.2 °C, 27.9-32.1 °C and 30.2-34.4 °C respectively. Of those, 'Renong No. 1' could maintain relatively high pollen germination rate even at 30 °C, however, 'Deshehari' had the narrowest adaptive temperature range. These results were further confirmed by changes of superoxide dismutase, catalase activity and malondialdehyde content. These results showed that mango flowering was highly sensitive to temperature changes and there were significant differences in pollen germination rate and pollen tube length among different varieties. Current research results were of great significance for the introduction of new mango varieties in different ecological regions, the cultivation and management of mango at the flowering stage and the breeding of new mango varieties.