Early reoxygenation in tumors after irradiation: Determining factors and consequences for radiotherapy regimens using daily multiple fractions

• Published in: International journal of radiation oncology, biology, physics Vol. 63; no. 3; pp. 901 - 910
• Main Authors: Crokart, Nathalie, Jordan, Bénédicte F., Baudelet, Christine, Ansiaux, Reginald, Sonveaux, Pierre, Grégoire, Vincent, Beghein, Nelson, DeWever, Julie, Bouzin, Caroline, Feron, Olivier, Gallez, Bernard
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2005
• Subjects: Animals; Carcinoma, Hepatocellular - blood supply; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - radiotherapy; Dose Fractionation; Electron paramagnetic resonance; Extracellular Fluid - physiology; Fibrosarcoma - blood supply; Fibrosarcoma - metabolism; Fibrosarcoma - radiotherapy; Liver Neoplasms - blood supply; Liver Neoplasms - metabolism; Liver Neoplasms - radiotherapy; Magnetic resonance imaging; Male; Mice; Mice, Inbred C3H; Neoplasms - blood supply; Neoplasms - metabolism; Neoplasms - radiotherapy; Nitric Oxide Synthase Type III - metabolism; Oximetry - methods; Oxygen Consumption - physiology; Oxygen Consumption - radiation effects; Partial Pressure; Radiation Tolerance; Radiotherapy; Time Factors; Tumor microenvironment; Tumor oxygenation; Electron paramagnetic resonance; Magnetic resonance imaging; Tumor microenvironment; Radiotherapy; Tumor oxygenation
• ISSN: 0360-3016; 1879-355X
• DOI: 10.1016/j.ijrobp.2005.02.038

Purpose: To characterize changes in the tumor microenvironment early after irradiation and determine the factors responsible for early reoxygenation. Methods and Materials: Fibrosarcoma type II (FSaII) and hepatocarcinoma transplantable liver tumor tumor oxygenation were determined using electron paramagnetic resonance oximetry and a fiberoptic device. Perfusion was assessed by laser Doppler, dynamic contrast-enhanced MRI, and dye penetration. Oxygen consumption was determined by electron paramagnetic resonance. The interstitial fluid pressure was evaluated by the wick-in-needle technique. Results: An increase in oxygen partial pressure was observed 3–4 h after irradiation. This increase resulted from a decrease in global oxygen consumption and an increase in oxygen delivery. The increase in oxygen delivery was due to radiation-induced acute inflammation (that was partially inhibited by the antiinflammatory agent diclofenac) and to a decrease in interstitial fluid pressure. The endothelial nitric oxide synthase pathway, identified as a contributing factor at 24 h after irradiation, did not play a role in the early stage after irradiation. We also observed that splitting a treatment of 18 Gy into two fractions separated by 4 h (time of maximal reoxygenation) had a greater effect on tumor regrowth delay than when applied as a single dose. Conclusion: Although the cell cycle redistribution effect is important for treatment protocols using multiple daily radiation fractions, the results of this work emphasize that the oxygen effect must be also considered to optimize the treatment strategy.