Rapid in-EPON CLEM: Combining fast and efficient labeling of self-labeling enzyme tags with EM-resistant Janelia Fluor dyes and StayGold

• Published in: Heliyon Vol. 10; no. 7; p. e28055
• Main Authors: Franzkoch, Rico, Wilkening, Sabrina, Liss, Viktoria, Holtmannspötter, Michael, Kurre, Rainer, Psathaki, Olympia E., Hensel, Michael
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15.04.2024; Elsevier
• Subjects: HaloTag; In-resin correlative light and electron microscopy; Janelia fluor dyes; Post-embedding CLEM; Self-labeling enzyme tags; Self-labeling enzyme tags; In-resin correlative light and electron microscopy; HaloTag; Janelia fluor dyes; Post-embedding CLEM
• ISSN: 2405-8440; 2405-8440
• DOI: 10.1016/j.heliyon.2024.e28055

Correlative light and electron microscopy (CLEM) combines light microscopy (LM) of fluorescent samples to ultrastructural analyses by electron microscopy (EM). Pre-embedding CLEM often suffers from inaccurate correlation between LM and EM modalities. Post-embedding CLEM enables precise registration of structures directly on EM sections, but requires fluorescent markers withstanding EM sample preparation, especially osmium tetroxide fixation, dehydration and EPON embedding. Most fluorescent proteins (FPs) lose their fluorescence during such conventional embedding (CE), but synthetic dyes represent promising alternatives as their stability exceeds those of FP. We analyzed various Janelia Fluor dyes and TMR conjugated to ligands for self-labeling enzymes, such as HaloTag, for fluorescence preservation after CE. We show that TMR, JF525, JF549, JFX549 and JFX554 retain fluorescence, with JFX549 and JFX554 yielding best results overall, also allowing integration of high-pressure freezing and freeze substitution. Furthermore, we found the recently published FP StayGold to resist CE, facilitating dual-fluorescence in-resin CLEM.