The Association Between the Interleukin-1 Polymorphisms and Gastric Cancer Risk Depends on the Family History of Gastric Carcinoma in the Study Population

• Published in: The American journal of gastroenterology Vol. 101; no. 2; pp. 248 - 254
• Main Authors: STARZYNSKA, Teresa, FERENC, Katarzyna, WEX, Thomas, KÄHNE, Thilo, LUBINSKI, Jan, LAWNICZAK, Malgorzata, MARLICZ, Krzysztof, MALFERTHEINER, Peter
• Format: Journal Article
• Language: English
• Published: Oxford Blackwell Publishing 01.02.2006; Wolters Kluwer Health Medical Research, Lippincott Williams & Wilkins
• Subjects: Adult; Aged; Aged, 80 and over; Biological and medical sciences; Carcinoma - blood; Carcinoma - epidemiology; Carcinoma - genetics; DNA, Neoplasm - genetics; Duodenal Ulcer - blood; Duodenal Ulcer - genetics; Electrophoresis, Agar Gel; Female; Gastroenterology; Gastroenterology. Liver. Pancreas. Abdomen; Genetic Predisposition to Disease; Genotype; Helicobacter pylori; Humans; Interleukin 1 Receptor Antagonist Protein; Interleukin-1 - blood; Interleukin-1 - genetics; Introns; Male; Mass Spectrometry; Medical sciences; Middle Aged; Polymerase Chain Reaction; Polymorphism, Genetic; Prevalence; Prospective Studies; Receptors, Interleukin-1 - antagonists & inhibitors; Risk Factors; Sialoglycoproteins - blood; Sialoglycoproteins - genetics; Stomach Neoplasms - blood; Stomach Neoplasms - epidemiology; Stomach Neoplasms - genetics; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Tumors; Stomach carcinoma; Family study; Risk factor; Gastroenterology; Interleukin 1; Digestive diseases; Malignant tumor; Stomach cancer; Gastric disease; Polymorphism
• ISSN: 0002-9270; 1572-0241
• DOI: 10.1111/j.1572-0241.2006.00422.x

The association between interleukin-1 polymorphisms, H. pylori and increased gastric cancer risk remains controversial. To compare the prevalence of these polymorphisms in individuals with two mutually exclusive diseases connected with infection, gastric cancer, and duodenal ulcer. 121 gastric cancer and 119 duodenal ulcer patients. Genomic DNA was typed for polymorphisms at position -511, -31 in the interleukin-1beta gene (IL-1 beta) using primer extension and mass-spectrometry. Analysis of the variable number of tandem repeats in intron 2, in its receptor antagonist gene (IL-1RN) was performed by PCR and agarose gel electrophoresis. All subjects were successfully genotyped for the three gene loci. IL-1 beta-511 was found to be in reverse linkage disequilibrium with IL-1 beta-31. The differences between gastric cancer and duodenal ulcer patients concerned only heterozygous variant of IL-1beta and were related to family history of gastric cancer, tumor stage, histology, site. Thus, CT carriers were found to have a higher risk of sporadic [OR 2.21 (95% CI, 1.22-3.99)], early [OR 2.81 (95% CI, 1.14-6.93)], diffuse [OR 2.48, (95% CI 1.21-5.09)] or non-cardia gastric cancer [OR 1.88 (95% CI 1.06-3.33)]. Furthermore, CT genotype was significantly more prevalent in gastric cancer patients with negative than in those with a positive family history (p = 0.039). The association between the interleukin-1 polymorphisms and gastric cancer risk depends on the family history of gastric carcinoma in the study population. This phenomenon may be in part responsible for differences in results of interleukin-1 studies performed on populations with low and high gastric cancer prevalence.