The metal binding site of the major house dust mite allergen Der p 1

Enzyme kinetic measurements on Der p 1 are usually performed in the presence of EDTA to prevent cation-facilitated inactivation of the active site cysteine residue, and therefore the metal binding site is unlikely to be occupied under these conditions.

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Bibliographic Details
Published inJournal of allergy and clinical immunology Vol. 118; no. 4; p. 971
Main Authors Meno, Kåre, Thorsted, Peter B., Ipsen, Henrik, Kristensen, Ole, Larsen, Jørgen N., Spangfort, Michael D., Gajhede, Michael, Lund, Kaare
Format Journal Article
LanguageEnglish
Published United States Mosby, Inc 01.10.2006
Elsevier Limited
Subjects
Animals
Antigens, Dermatophagoides - chemistry
Antigens, Dermatophagoides - metabolism
Arthropod Proteins
Binding Sites
Crystal structure
Crystallization
Cysteine Endopeptidases
Enzyme kinetics
Magnesium
Metals - metabolism
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Summary:Enzyme kinetic measurements on Der p 1 are usually performed in the presence of EDTA to prevent cation-facilitated inactivation of the active site cysteine residue, and therefore the metal binding site is unlikely to be occupied under these conditions.
Bibliography:SourceType-Other Sources-1
content type line 63
ObjectType-Correspondence-1
ObjectType-Commentary-2
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2006.06.038