Heterologous Expression of Two Brassica campestris CCCH Zinc-Finger Proteins in Arabidopsis Induces Cytoplasmic Foci and Causes Pollen Abortion

The membrane-less organelles in cytoplasm that are presented as cytoplasmic foci were successively identified. Although multiple CCCH zinc-finger proteins have been found to be localized in cytoplasmic foci, the relationship between their specific localization and functions still needs further clari...

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Published inInternational journal of molecular sciences Vol. 24; no. 23; p. 16862
Main Authors Xu, Liai, Xiong, Xingpeng, Liu, Tingting, Cao, Jiashu, Yu, Youjian
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 01.12.2023
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Summary:The membrane-less organelles in cytoplasm that are presented as cytoplasmic foci were successively identified. Although multiple CCCH zinc-finger proteins have been found to be localized in cytoplasmic foci, the relationship between their specific localization and functions still needs further clarification. Here, we report that the heterologous expression of two CCCH zinc-finger protein genes ( and ) in can affect microgametogenesis by involving the formation of cytoplasmic foci. By monitoring the distribution of proteins and observing pollen phenotypes, we found that, when these two proteins were moderately expressed in pollen, they were mainly dispersed in the cytoplasm, and the pollen developed normally. However, high expression induced the assembly of cytoplasmic foci, leading to pollen abortion. These findings suggested that the continuous formation of BcMF30a/BcMF30c-associated cytoplasmic foci due to high expression was the inducement of male sterility. A co-localization analysis further showed that these two proteins can be recruited into two well-studied cytoplasmic foci, processing bodies (PBs), and stress granules (SGs), which were confirmed to function in mRNA metabolism. Together, our data suggested that BcMF30a and BcMF30c play component roles in the assembly of pollen cytoplasmic foci. Combined with our previous study on the homologous gene of in , we concluded that the function of these homologous genes is conserved and that cytoplasmic foci containing may participate in the regulation of gene expression in pollen by regulating mRNA metabolism.
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ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms242316862