Simultaneous determination of the levels of deoxynivalenol, 3-acetyldeoxynivalenol, and nivalenol in grain and feed samples from South Korea using a high-performance liquid chromatography–photodiode array detector

• Published in: Applied biological chemistry Vol. 59; no. 6; pp. 881 - 887
• Main Authors: Kim, Dong-Ho, Hong, Sung-Yong, Jeon, Mi-Hyeon, An, Jae-Min, Kim, Sung-Youn, Kim, Hyo-Young, Yoon, Bo Ryun, Chung, Soo Hyun
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.12.2016; Springer Nature B.V; 한국응용생명화학회
• Subjects: Applied Microbiology; Biological Techniques; Bioorganic Chemistry; Chemistry; Chemistry and Materials Science; Deoxynivalenol; Feeds; Grain; High performance liquid chromatography; Liquid chromatography; Nivalenol; Photodiodes; 농학; South Korea; 3-Acetyldeoxynivalenol; Nivalenol; Deoxynivalenol; Grain; Feed
• ISSN: 2468-0834; 2468-0842
• DOI: 10.1007/s13765-016-0238-8

An HPLC method combined with immunoaffinity clean-up was developed for the simultaneous analysis of deoxynivalenol (DON), nivalenol (NIV), and 3-acetyldeoxynivalenol (3AcDON) in grains and feeds. The limits of quantification of the method for DON, 3AcDON, and NIV were 11.0, 27.6, and 13.5 ng/g, respectively. HPLC analyses of 300 samples of grains and feeds collected in South Korea showed that the incidence of DON and NIV in corn was 22.5 and 7.5%, while that in rice was 4.0 and 54.0%, respectively. The incidence of DON and NIV in mixed grain powder was 62.0 and 24.0%, respectively. Our study also showed that animal feeds were mainly contaminated with DON (98.1%) in the range of 32.8–950.25 ng/g with the mean concentration of 353.32 ng/g. The levels of DON in grains did not exceed the maximum allowable limit (1 mg/kg) set by the Korean Food and Drug Administration.